Cross-Disciplinary Glucose Biosensors: An ORMOSIL/Enzyme Material for Enhanced Detection

Cross-Disciplinary Glucose Biosensors: An ORMOSIL/Enzyme Material for Enhanced Detection Full article

Journal

ACS Applied Polymer Materials
ISSN: 2637-6105

Output data

Year: 2024, Volume: 6, Number: 20, Pages: 12405–12419 Pages count : DOI: 10.1021/acsapm.4c01394

Authors

Kuznetsova Lyubov S. ¹ , Ivanova Kristina D. ¹ , Lantsova Elizaveta A. ¹ , Saverina Evgeniya A. ¹ , Kharkova Anna S. ¹ , Guetnga Christian ¹ , Lipkin Michael S. ² , Kamanina Olga A. ¹ , Kozlova Tatyana V. ¹ , Popova Nadezhda M. ³ , Savishcheva Anna A. ⁴ , Losev Timofey V. ^5,6 , Bekmansurov Danis R. ⁶ , Chaliy Vasiliy A. ⁶ , Medvedev Michael G. ^6,1 , Vereshchagin Anatoly N. ⁶ , Arlyapov Vyacheslav A. ¹ , Ananikov Valentine P. ^6,7

Affiliations

1	Tula State University, Lenina Pr., 92, Tula 300012, Russia
2	Platov South-Russian State Polytechnic University (NPI), Prosvescheniya St., 132, Novocherkassk 346428, Russia
3	Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, Leninsky Pr., 31 k. 4, Moscow 119071, Russia
4	GOZ TO “Tula Regional Clinical Hospital″, Yablochkov St., 1, Tula 300053, Russia
5	Lomonosov Moscow State University, Leninskie Gory 1/3, Moscow 119991, Russia
6	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Pr., 47, Moscow 119991, Russia
7	Organic Chemistry Department, RUDN University, 6 Miklukho-Maklaya St, Moscow 117198, Russia

In this study, we introduce an efficient approach to cross-disciplinary glucose biosensor technology through the development of hybrid nanocomposite materials. These materials were crafted from redox-active polymers embedded in silica sol–gel matrices, intricately linked with phenazine mediators and reinforced with carbon nanotubes. By leveraging advanced analytical techniques, including NMR spectroscopy, scanning electron microscopy, and confocal microscopy, we characterized the structures of these redox-active polymers. Our investigation further addressed their electrochemical behaviors by employing cyclic voltammetry and impedance spectroscopy to elucidate their distinctive properties. Employing a complex analytical strategy and a computational approach, this study identified an optimal redox-active system that shows synergy between multiwalled nanotubes and engineered redox-active polymers. This polymer, which is composed of (3-aminopropyl)triethoxysilane and tetraethoxysilane at an optimized ratio of 20:80 vol %, is seamlessly integrated with a covalently bonded neutral red mediator. The resulting biosensor is capable of detecting glucose across a range of 0.01–0.92 mM with a low detection limit of 0.003 mM. Its operational stability is 1.9%, coupled with an unparalleled selectivity that holds promise for further enhancement through machine learning techniques. This machine learning breakthrough represents a significant leap forward in the accurate quantification of glucose in diverse samples, achieving a high degree of correlation with established methods. The composite material revealed in this research has implications for further applications in biosensing technology. The biocompatibility, nontoxicity, stability, and superior conductivity of the material underscore its potential in the field, opening possibilities for the development of blood glucose measurement techniques.

Kuznetsova L.S. , Ivanova K.D. , Lantsova E.A. , Saverina E.A. , Kharkova A.S. , Guetnga C. , Lipkin M.S. , Kamanina O.A. , Kozlova T.V. , Popova N.M. , Savishcheva A.A. , Losev T.V. , Bekmansurov D.R. , Chaliy V.A. , Medvedev M.G. , Vereshchagin A.N. , Arlyapov V.A. , Ananikov V.P.
Cross-Disciplinary Glucose Biosensors: An ORMOSIL/Enzyme Material for Enhanced Detection
ACS Applied Polymer Materials. 2024. V.6. N20. P.12405–12419. DOI: 10.1021/acsapm.4c01394 WOS OpenAlex

Web of science:	WOS:001333375500001
OpenAlex:	W4403423996

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