Protein - carbohydrate interaction studies using domestic animals as role models support the search of new glycomimetic molecules

Protein - carbohydrate interaction studies using domestic animals as role models support the search of new glycomimetic molecules Full article

Journal

International Journal of Biological Macromolecules
ISSN: 1879-0003 , E-ISSN: 0141-8130

Output data

Year: 2024, Volume: 279, Article number : 134951, Pages count : DOI: 10.1016/j.ijbiomac.2024.134951

Authors

Zhang Ning ¹ , Li Lan ² , Mohri Marzieh ² , Siebert Simone ² , Lütteke Thomas ³ , Louton Helen ⁴ , Bednarikova Zuzana ⁵ , Gazova Zuzana ⁵ , Nifantiev Nikolay ⁶ , Jandowsky Anabell ⁷ , Frölich Kai ⁷ , Eckert Thomas ⁸ , Loers Gabriele ⁹ , Petridis Athanasios K ¹⁰ , Bhunia Anirban ¹¹ , Mohid Sk Abdul ¹¹ , Scheidig Axel J ¹² , Liu Guiqin ¹³ , Zhang Ruiyan ¹³ , Lochnit Günter ¹⁴ , Siebert Hans-Christian ¹⁵

Affiliations

1	Institute of BioPharmaceutical Research, Liaocheng University, Liaocheng 252059, China. Electronic address: zhangning1111@126.com.
2	RI-B-NT - Research Institute of Bioinformatics and Nanotechnology, Schauenburgerstr. 116, 24118 Kiel, Germany.
3	Institut für Veterinärphysiologie und Biochemie, Fachbereich Veterinärmedizin, Justus-Liebig-Universität Gießen, Frankfurter Str. 100, 35392 Gießen, Germany.
4	Animal Health and Animal Welfare, Faculty of Agricultural and Environmental Sciences, University of Rostock, Justus-von-Liebig-Weg 6b, 18059 Rostock, Germany.
5	Department of Biophysics, Institute of Experimental Physics, Slovak Academy of Sciences, Watsonova 47, 04001, Kosice, Slovakia.
6	Laboratory of Glycoconjugate Chemistry, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, 119991 Moscow, Russia.
7	Tierpark Arche Warder, Zentrum für seltene Nutztierrassen e. V., Langwedeler Weg 11, 24646 Warder, Germany.
8	Department of Chemistry and Biology, University of Applied Sciences Fresenius, Limburger Str. 2, 65510 Idstein, Germany; RISCC Research Institute for Scientific Computing and Consulting, Heuchelheim, Germany.
9	Center for Molecular Neurobiology Hamburg, University Medical Center, Hamburg-Eppendorf, University of Hamburg, Falkenried 94, 20251 Hamburg, Germany.
10	Medical School, Heinrich-Heine-Universität Düsseldorf, Department of Neurosurgery, St. Lukes Hospital, Thessaloniki, Greece.
11	Department of Chemical Sciences, Bose Institute, Unified Academic Campus, Sector V, EN 80, Kolkata 700091, India.
12	Zoological Institute, Department of Structural Biology, Kiel University, Am Botanischen Garten 1-9, 24118 Kiel, Germany.
13	Institute of BioPharmaceutical Research, Liaocheng University, Liaocheng 252059, China.
14	Institut für Biochemie, Fachbereich Humanmedizin, Justus-Liebig-Universität Gießen, Friedrichstrasse 24, 35390 Gießen, Germany.
15	RI-B-NT - Research Institute of Bioinformatics and Nanotechnology, Schauenburgerstr. 116, 24118 Kiel, Germany. Electronic address: hcsiebert@aol.com.

The structural dynamics of the interactions between defensins or lysozymes and various saccharide chains that are covalently linked to lipids or proteins were analyzed in relation to the sub-molecular architecture of the carbohydrate binding sites of lectins. Using tissue materials from rare and endangered domestic animals as well as from dogs it was possible to compare these results with data obtained from a human glioblastoma tissue. The binding mechanisms were analyzed on a cellular and a sub-molecular size level using biophysical techniques (e.g. NMR, AFM, MS) which are supported by molecular modeling tools. This leads to characteristic structural patterns being helpful to understand glyco-biochemical pathways in which galectins, defensins or lysozymes are involved. Carbohydrate chains have a distinct impact on cell differentiation, cell migration and immunological processes. The absence or the presence of sialic acids and the conformational dynamics in glycans are often correlated with zoonoses such as influenza- and coronavirus-infections. Receptor-sensitive glycomimetics could be a solution. The new findings concerning the function of galectin-3 in the nucleus in relation to differentiation processes can be understood when the binding specificity of neuroleptic molecules as well as the interactions between proteins and nucleic acids are describable on a sub-molecular size level.

Zhang N. , Li L. , Mohri M. , Siebert S. , Lütteke T. , Louton H. , Bednarikova Z. , Gazova Z. , Nifantiev N. , Jandowsky A. , Frölich K. , Eckert T. , Loers G. , Petridis A.K. , Bhunia A. , Mohid S.A. , Scheidig A.J. , Liu G. , Zhang R. , Lochnit G. , Siebert H-C.
Protein - carbohydrate interaction studies using domestic animals as role models support the search of new glycomimetic molecules
International Journal of Biological Macromolecules. 2024. V.279. 134951 . DOI: 10.1016/j.ijbiomac.2024.134951 WOS Scopus OpenAlex

≡ Web of science:	WOS:001312900600001
≡ Scopus:	2-s2.0-85202541217
≡ OpenAlex:	W4401753451