An Acinetobacter baumannii nasal carriage isolate recovered from an asymptomatic patient in Vietnam is extensively antibiotic resistant and produces a rare K71 type capsule

An Acinetobacter baumannii nasal carriage isolate recovered from an asymptomatic patient in Vietnam is extensively antibiotic resistant and produces a rare K71 type capsule Full article

Journal

Microbiology spectrum
ISSN: 2165-0497

Output data

Year: 2024, Volume: 12, Number: 12, Article number : е01838-24, Pages count : DOI: 10.1128/spectrum.01838-24

Authors

Shpirt Anna M. ¹ , Harmer Christopher J. ² , Shashkov Alexander S. ¹ , Shneider Mikhail M. ³ , Chizhov Alexander O. ¹ , Dmitrenok Andrey S. ¹ , Popova Anastasiya V. ⁴ , Kasimova Anastasiya A. ¹ , Perepelov Andrei V. ¹ , Knirel Yuriy A. ¹ , Hall Ruth M. ² , Kenyon Johanna J. ⁵

Affiliations

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
2	School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia
3	M. M. Shemyakin and Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
4	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia
5	School of Pharmacy and Medical Sciences, Health Group, Griffith University, Gold Coast Campus, Southport, Queensland, Australia

Acinetobacter baumannii is a leading cause of multidrug-resistant bacterial infections worldwide, and the capsular polysaccharide (CPS) is a major virulence determinant. A previous study of A. baumannii from intubated and asymptomatic patients admitted to the intensive care unit (ICU) at the Hospital for Tropical Diseases in Ho Chi Minh City in Vietnam revealed multiple lineages with diverse antibiotic resistance profiles and CPS biosynthesis loci. Here, we show that 48_n, an asymptomatic nasal carriage isolate belonging to ST142, is extensively antibiotic resistant and carries acquired resistance determinants accounting for the resistance profile. 48_n carries the novel KL71 CPS biosynthesis locus in the chromosome. The structure of the CPS produced by 48_n was established using 1H and 13C nuclear magnetic resonance spectroscopy, including two-dimensional 1Н,1Н COSY, 1Н,1Н TOCSY, 1Н,1Н ROESY, 1Н,13C HSQC, and 1Н,13C HMBC experiments, and confirmed by Smith degradation. Consistent with the genetic content of KL71, the K71 CPS was found to be made up of octasaccharide K units containing six l-rhamnose residues and one residue each of N-acetyl-d-glucosamine and d-glucuronic acid. K71 CPS was branched and closely related to the K74 CPS produced by BAL_309, an antibiotic susceptible ST142 isolate recovered from an intubated patient in the same ICU 7 years later. K71 and K74 differ only in the linkage between K units, and this is due to the replacement of a single gene at the K locus that codes for the Wzy polymerase.

Shpirt A.M. , Harmer C.J. , Shashkov A.S. , Shneider M.M. , Chizhov A.O. , Dmitrenok A.S. , Popova A.V. , Kasimova A.A. , Perepelov A.V. , Knirel Y.A. , Hall R.M. , Kenyon J.J.
An Acinetobacter baumannii nasal carriage isolate recovered from an asymptomatic patient in Vietnam is extensively antibiotic resistant and produces a rare K71 type capsule
Microbiology spectrum. 2024. V.12. N12. е01838-24 . DOI: 10.1128/spectrum.01838-24 WOS OpenAlex

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