Structural characterization of arabinogalactan-II and pectin from Urtica cannabina

Structural characterization of arabinogalactan-II and pectin from Urtica cannabina Full article

Journal

Carbohydrate Polymers
ISSN: 1879-1344 , E-ISSN: 0144-8617

Output data

Year: 2025, Volume: 348, Article number : 122868, Pages count : DOI: 10.1016/j.carbpol.2024.122868

Authors

Golovchenko Victoria V ¹ , Khlopin Victor A ¹ , Patova Olga A ¹ , Vityazev Fedor V ¹ , Dmitrenok Andrey S ² , Shashkov Alexander S ²

Affiliations

1	Institute of Physiology of Komi Science Centre of The Urals Branch of the Russian Academy of Sciences, FRC Komi SC UB RAS, 50, Pervomaiskaya str., 167982 Syktyvkar, Russia. Electronic address: lemnan@mail.ru.
2	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47, Leninsky prospect, Moscow 119991, Russia.

Comparative analysis of extracellular and cell wall glycans from Urtica cannabina leaves was performed using chemical methods, GC, GC–MS, 1D, and 2D NMR spectroscopy. The structures of extracellular AG-II and cell wall AG-II are similar. The units are typical for AG-IIs: β-GlcpA-4-OMe-(1→, Rhap-(1 → 4)-β-GlcpA-(1→, attached to β-Galp at O-6, as well as arabinan chains attached to β-Galp at O-3. A single Araf and a trisaccharide formed by 2,5-Araf and two terminal Araf form short arabinan side chains in AG-II. 1,5-arabinan with a backbone substituted by a single Araf at O-3 was identified only in the side chains of cell wall AG-II. The side chains can be attached to O-3 and O-6 of the same β-Galp to form a bifurcated AG side chain. The backbone of AG-II is formed by 1,6- rather than 1,3-linked Galp, although it does include some 1,3-Galp. The high content of 3,6-Galp shows the highly branched nature of the AG carbohydrate chains. From the cell wall, AGP was extracted together with pectin, the simultaneous elution of which from both DEAE-cellulose and Sepharose may indicate a link between them.

Golovchenko V.V. , Khlopin V.A. , Patova O.A. , Vityazev F.V. , Dmitrenok A.S. , Shashkov A.S.
Structural characterization of arabinogalactan-II and pectin from Urtica cannabina
Carbohydrate Polymers. 2025. V.348. 122868 . DOI: 10.1016/j.carbpol.2024.122868 WOS OpenAlex

Web of science:	WOS:001338591300001
OpenAlex:	W4403374734

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