The unique structure of bacterial polysaccharides - Immunochemical studies on the O-antigen of Proteus penneri 4034-85 clinical strain classified into a new O83 Proteus serogroup

The unique structure of bacterial polysaccharides - Immunochemical studies on the O-antigen of Proteus penneri 4034-85 clinical strain classified into a new O83 Proteus serogroup Full article

Journal

International Journal of Biological Macromolecules
ISSN: 1879-0003 , E-ISSN: 0141-8130

Output data

Year: 2020, Volume: 163, Pages: 1168-1174 Pages count : 7 DOI: 10.1016/j.ijbiomac.2020.07.012

Authors

Siwińska Małgorzata ¹ , Zabłotni Agnieszka ¹ , Levina Evgeniya A. ^2,3 , Shashkov Alexander S. ³ , Ovchinnikova Olga G. ³ , Różalski Antoni ¹ , Knirel Yuriy A. ³

Affiliations

1	Department of Biology of Bacteria, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland
2	Higher Chemical College of the Russian Academy of Sciences, 125047 Moscow, Russia
3	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia

The serological classification scheme of the opportunistic Proteus bacilli includes a number of Proteus penneri strains. The tested P. penneri 4034-85 strain turned out to be serologically distinguished in ELISA and Western blotting. The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of this strain and studied by sugar and methylation analyses and dephosphorylation along with 1H and 13C NMR spectroscopy, including 2D 1H,1H COSY, TOCSY, ROESY, 1H,13C HSQC, HMBC, and HSQC-TOCSY experiments, The O-polysaccharide was found to have a linear repeating unit containing glycerol 1-phosphate and two residues each of Gal and GlcNAc. The following O-polysaccharide structure was established, which, to our knowledge, is unique among known bacterial polysaccharide structures: →4)-β-d-GlcpNAc-(1→3)-α-d-Galp-(1→3)-β-d-GlcpNAc-(1→2)-β-d-Galp-(1→3)-Gro-1-P-(O→.

Siwińska M. , Zabłotni A. , Levina E.A. , Shashkov A.S. , Ovchinnikova O.G. , Różalski A. , Knirel Y.A.
The unique structure of bacterial polysaccharides - Immunochemical studies on the O-antigen of Proteus penneri 4034-85 clinical strain classified into a new O83 Proteus serogroup
International Journal of Biological Macromolecules. 2020. V.163. P.1168-1174. DOI: 10.1016/j.ijbiomac.2020.07.012 WOS Scopus OpenAlex

Web of science:	WOS:000579839600117
Scopus:	2-s2.0-85088093591
OpenAlex:	W3041802107

DB	Citing
OpenAlex	14
Scopus	13
Web of science	13