Escherichia albertii EA046 (O9) harbors two polysaccharide gene clusters for synthesis of the O-antigen by the Wzx/Wzy-dependent pathway and a mannan shared by Escherichia coli O8 by the Wzm/Wzt-dependent pathway

Escherichia albertii EA046 (O9) harbors two polysaccharide gene clusters for synthesis of the O-antigen by the Wzx/Wzy-dependent pathway and a mannan shared by Escherichia coli O8 by the Wzm/Wzt-dependent pathway Full article

Journal

International Journal of Biological Macromolecules
ISSN: 1879-0003 , E-ISSN: 0141-8130

Output data

Year: 2020, Volume: 142, Pages: 609-614 Pages count : 6 DOI: 10.1016/j.ijbiomac.2019.09.135

Authors

Naumenko Olesya I. ¹ , Zheng Han ² , Shashkov Alexander S. ¹ , Sun Yong ³ , Senchenkova Sof'ya N. ¹ , Bai Li ⁴ , Wang Jianping ² , Wang Hong ⁵ , Li Qun ⁵ , Knirel Yuriy A. ¹ , Xiong Yanwen ^6,2

Affiliations

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
2	State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
3	Anhui Provincial Center for Disease Control and Prevention, Hefei, Anhui Province, China
4	Division I of Risk Assessment, China National Center for Food Safety Risk Assessment, Beijing, China
5	Zigong Center for Disease Control and Prevention, Zigong, Sichuan Province, China
6	Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang Province, China

O antigen is a polysaccharide chain of a lipopolysaccharide on the outer membrane of Gram-negative bacteria. O-antigen-based serotyping and molecular typing are widely used for epidemiological and surveillance purposes. Two polysaccharides were isolated by Sephadex G-50 gel-permeation chromatography following mild acid degradation of the lipopolysaccharide of Escherichia albertii EA046 assigned to serotype O9. The polysaccharide eluted first was considered as the O-antigen. It was composed of tetrasaccharide repeating units containing two residues of d-Man and one residue each of d-Gal and d-GlcNAc as well as glycerol phosphate. It had the following unique structure which was established by NMR spectroscopy applied to the initial and dephosphorylated polysaccharides: The polysaccharide eluted from the gel second was identified as a mannan with a → 3)-β-d-Manp-(1 → 2)-α-d-Manp-(1 → 2)-α-d-Manp-(1 → trisaccharide repeating unit. In E. albertii EA046, two polysaccharide gene clusters were found at a chromosomal locus flanked by the conserved galF gene and the histidine synthesis operon (his). They were suggested to drive the biosynthesis of the O-antigen by the Wzy/Wzy-dependent pathway and the mannan by the Wzm/Wzt-dependent pathway. The mannan shares the structure and gene cluster with a polysaccharide isolated earlier from the lipopolysaccharide of Escherichia coli O8.

Naumenko O.I. , Zheng H. , Shashkov A.S. , Sun Y. , Senchenkova S.N. , Bai L. , Wang J. , Wang H. , Li Q. , Knirel Y.A. , Xiong Y.
Escherichia albertii EA046 (O9) harbors two polysaccharide gene clusters for synthesis of the O-antigen by the Wzx/Wzy-dependent pathway and a mannan shared by Escherichia coli O8 by the Wzm/Wzt-dependent pathway
International Journal of Biological Macromolecules. 2020. V.142. P.609-614. DOI: 10.1016/j.ijbiomac.2019.09.135 WOS Scopus OpenAlex

Web of science:	WOS:000510955500060
Scopus:	2-s2.0-85075376047
OpenAlex:	W2980780771

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