Structure, gene cluster of the O antigen and biological activity of the lipopolysaccharide from the rhizospheric bacterium Ochrobactrum cytisi IPA7.2

Structure, gene cluster of the O antigen and biological activity of the lipopolysaccharide from the rhizospheric bacterium Ochrobactrum cytisi IPA7.2 Full article

Journal

International Journal of Biological Macromolecules
ISSN: 1879-0003 , E-ISSN: 0141-8130

Output data

Year: 2020, Volume: 154, Pages: 1375-1381 Pages count : 7 DOI: 10.1016/j.ijbiomac.2019.11.017

Authors

Sigida Elena N. ¹ , Kargapolova Kristina Y. ² , Shashkov Alexander S. ³ , Zdorovenko Evelina L. ³ , Ponomaryova Tatyana S. ⁴ , Meshcheryakova Anna A. ⁴ , Tkachenko Oksana V. ² , Burygin Gennady L. ^5,2 , Knirel Yuriy A. ³

Affiliations

1	Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, 410049, Russia.
2	Vavilov Saratov State Agrarian University, Saratov 410012, Russia
3	Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia
4	Chernyshevsky Saratov State University, Saratov 410012, Russia
5	Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov 410049, Russia

Lipopolysaccharide (LPS) of Ochrobactrum cytisi IPA7.2, a bacterium isolated from the roots of Solanum tuberosum L., was extracted from dry bacterial cells and chemically characterized. The O-specific polysaccharide was obtained by mild acid hydrolysis of the LPS and studied by sugar analysis and 1H and 13C NMR spectroscopy, including 1H,1H COSY, 1H,1H TOCSY, 1H,1H ROESY, 1H,13C HSQC, and 1H,13C HMBC experiments. The polysaccharide was linear and consisted of trisaccharide repeating units of the following structure:

Sigida E.N. , Kargapolova K.Y. , Shashkov A.S. , Zdorovenko E.L. , Ponomaryova T.S. , Meshcheryakova A.A. , Tkachenko O.V. , Burygin G.L. , Knirel Y.A.
Structure, gene cluster of the O antigen and biological activity of the lipopolysaccharide from the rhizospheric bacterium Ochrobactrum cytisi IPA7.2
International Journal of Biological Macromolecules. 2020. V.154. P.1375-1381. DOI: 10.1016/j.ijbiomac.2019.11.017 WOS Scopus OpenAlex

Web of science:	WOS:000533508600134
Scopus:	2-s2.0-85076617113
OpenAlex:	W2991488338

DB	Citing
OpenAlex	14
Scopus	12
Web of science	10