Elucidation of the K32 Capsular Polysaccharide Structure and Characterization of the KL32 Gene Cluster of Acinetobacter baumannii LUH5549

Elucidation of the K32 Capsular Polysaccharide Structure and Characterization of the KL32 Gene Cluster of Acinetobacter baumannii LUH5549 Научная публикация

Журнал

Biochemistry-Moscow
ISSN: 0006-2979 , E-ISSN: 1608-3040

Вых. Данные

Год: 2020, Том: 85, Номер: 2, Страницы: 241-247 Страниц : 7 DOI: 10.1134/s000629792002011x

Авторы

Cahill S M ¹ , Arbatsky N P ² , Shashkov A S ² , Shneider M M ³ , Popova A V ⁴ , Hall R M ⁵ , Kenyon J J ¹ , Knirel Y A ²

Организации

1	Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, QLD 4001, Australia.
2	Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia
3	Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997 Russia.
4	Moscow Institute of Physics and Technology, 141701, Dolgoprudny, Moscow Region, Russia
5	School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia

Capsular polysaccharide (CPS), isolated from Acinetobacter baumannii LUH5549 carrying the KL32 capsule biosynthesis gene cluster, was studied by sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy. The K32 CPS was found to be composed of branched pentasaccharide repeats (K units) containing two residues of β-D-GalpNAc and one residue of β-D-GlcpA (β-D-glucuronic acid) in the main chain and one residue each of β-D-Glcp and α-D-GlcpNAc in the disaccharide side chain. Consistent with the established CPS structure, the KL32 gene cluster includes genes for a UDP-glucose 6-dehydrogenase (Ugd3) responsible for D-GlcA synthesis and four glyco- syltransferases that were assigned to specific linkages. Genes encoding an acetyltransferase and an unknown protein prod- uct were not involved in CPS biosynthesis. Whilst the KL32 gene cluster has previously been found in the global clone 2 (GC2) lineage, LUH5549 belongs to the sequence type ST354, thus demonstrating horizontal gene transfer between these lineages.

Cahill S.M. , Arbatsky N.P. , Shashkov A.S. , Shneider M.M. , Popova A.V. , Hall R.M. , Kenyon J.J. , Knirel Y.A.
Elucidation of the K32 Capsular Polysaccharide Structure and Characterization of the KL32 Gene Cluster of Acinetobacter baumannii LUH5549
Biochemistry-Moscow. 2020. V.85. N2. P.241-247. DOI: 10.1134/s000629792002011x WOS Scopus OpenAlex

Web of science:	WOS:000514515900011
Scopus:	2-s2.0-85079724106
OpenAlex:	W3008415894

БД	Цитирований
OpenAlex	13
Scopus	11
Web of science	9