Structure of O-Polysaccharide and Lipid A of Pantoea Agglomerans 8488

Structure of O-Polysaccharide and Lipid A of Pantoea Agglomerans 8488 Full article

Journal

Biomolecules
ISSN: 2218-273X

Output data

Year: 2020, Volume: 10, Number: 5, Article number : 804, Pages count : DOI: 10.3390/biom10050804

Authors

Bulyhina Tetiana V ¹ , Zdorovenko Evelina L ² , Varbanets Ludmila D ² , Shashkov Alexander S ² , Kadykova Alexandra A ³ , Knirel Yuriy A ² , Lushchak Oleh V ⁴

Affiliations

1	D.K. Zabolotny, Institute of Microbiology and Virology (IMV), the National Academy of Sciences, 154 Zabolotnoho Str., 03143 Kyiv, Ukraine.
2	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia
3	Higher Chemical College of the Russian Academy of Sciences, D.I. Mendeleev University of Chemical Technology of Russia, Moscow 125047, Russia
4	Department of Biochemistry and Biotechnology, Natural Sciences Institute, Vasyl Stefanyk Precarpathian National University, 76018 Ivano-Frankivsk, Ukraine

The Pantoea agglomerans 8488 lipopolysaccharide (LPS) was isolated, purified and characterized by monosaccharide and fatty acid analysis. The O-polysaccharide and lipid A components of the LPS were separated by mild acid degradation. Lipid A was studied by electrospray ionization mass spectrometry (ESI-MS) and found to consist of hexa-, penta-, tetra- and tri-acylated species. Two-dimensional nuclear magnetic resonance (NMR) spectroscopy revealed the following structure of the O-polysaccharide repeating unit →3)-α-L-Rhap-(1→6)-α-D-Manp-(1→3)-α-L-Fucp-(1→3)-β-D-GlcNAcp-(1→. The LPS showed a low level of toxicity, was not pyrogenic, and reduced the adhesiveness index of microorganisms to 2.12, which was twofold less than the control. LPS modified by complex compounds of germanium (IV) and tin (IV) were obtained. It was found that six LPS samples modified by Sn compounds and two LPS samples modified by Ge compounds lost their toxic activity when administered to mice in a dose of LD50 (105 µg/mice or 5 mg/kg). However, none of the modified LPS samples changed their serological activity in an Ouchterlony double immunodiffusion test in agar.

Bulyhina T.V. , Zdorovenko E.L. , Varbanets L.D. , Shashkov A.S. , Kadykova A.A. , Knirel Y.A. , Lushchak O.V.
Structure of O-Polysaccharide and Lipid A of Pantoea Agglomerans 8488
Biomolecules. 2020. V.10. N5. 804 . DOI: 10.3390/biom10050804 WOS Scopus OpenAlex

Web of science:	WOS:000545013700141
Scopus:	2-s2.0-85085277667
OpenAlex:	W3027892533

DB	Citing
OpenAlex	6
Scopus	6
Web of science	3