Hetero-Pathogenic O181:H4 EAHEC Strain of Sequence Type ST678 Associated with Hemolytic–Uremic Syndrome in Schoolchildren in Russia

Hetero-Pathogenic O181:H4 EAHEC Strain of Sequence Type ST678 Associated with Hemolytic–Uremic Syndrome in Schoolchildren in Russia Full article

Journal

Microorganisms
ISSN: 2076-2607

Output data

Year: 2023, Volume: 11, Number: 7, Article number : 1771, Pages count : DOI: 10.3390/microorganisms11071771

Authors

Kartsev Nikolay N. ¹ , Detusheva Elena V. ¹ , Kalmantaeva Olga V. ² , Korobova Olga V. ³ , Gerasimov Vladimir N. ⁴ , Kombarova Tatiana I. ³ , Borzilov Aleksander I. ³ , Fursova Nadezhda K. ¹ , Vereshchagin Anatoly N. ⁵ , Svetoch Edward A. ¹

Affiliations

1	Department of Molecular Microbiology, State Research Center for Applied Microbiology and Biotechnology, Territory “Kvartal A”, 142279 Obolensk, Russia
2	Laboratory of Molecular Biology, State Research Center for Applied Microbiology and Biotechnology, Territory “Kvartal A”, 142279 Obolensk, Russia
3	Laboratory of Biological Trials, State Research Center for Applied Microbiology and Biotechnology, Territory “Kvartal A”, 142279 Obolensk, Russia
4	Department of Disinfectology, State Research Center for Applied Microbiology and Biotechnology, Territory “Kvartal A”, 142279 Obolensk, Russia
5	N. D. Zelinsky Institute of Organic Chemistry, Leninsky pr. 47, 119991 Moscow, Russia

Background: In the last decade, the importance of hetero-pathogenic enteroaggregative Shiga-toxin-producing E. coli for public health has increased. Recently, we described the genetic background of the EAHEC O181:H4 strain of ST678 carrying the stx2 gene in prophage and five plasmids, including the plasmid-carrying aggR and aaiC genes. Here, we present the morphological and enzymatic characteristics of this strain, as well as susceptibility to antimicrobials, biofilm formation, etc. Methods: Bacterial morphology was studied using an electron microscope. Susceptibility to antimicrobials was determined using the microdilution method. Cytotoxicity was estimated in Vero cells. Virulence was studied on mice. Results: The morphological and enzymatic properties of the hetero-pathogenic EAHEC strain were typical for E. coli; electron microscopy revealed the specific flagella. The strain was susceptible to most antibiotics and disinfectants but resistant to ampicillin and ciprofloxacin and showed a high degree of biofilm formation. Cytotoxicity towards Vero cells was estimated as 80%. Conclusions: The emergence of a new O181:H4 EAHEC strain poses a potential threat to humans because of the virulence potential that must be taken into account in the epidemiological analysis of outbreaks and sporadic cases of foodborne infections associated with hemolytic–uremic syndrome.

Kartsev N.N. , Detusheva E.V. , Kalmantaeva O.V. , Korobova O.V. , Gerasimov V.N. , Kombarova T.I. , Borzilov A.I. , Fursova N.K. , Vereshchagin A.N. , Svetoch E.A.
Hetero-Pathogenic O181:H4 EAHEC Strain of Sequence Type ST678 Associated with Hemolytic–Uremic Syndrome in Schoolchildren in Russia
Microorganisms. 2023. V.11. N7. 1771 . DOI: 10.3390/microorganisms11071771 WOS Scopus OpenAlex

Web of science:	WOS:001036673300001
Scopus:	2-s2.0-85166266515
OpenAlex:	W4383723177

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