Abstract: Fungal mannans are polysaccharide antigens located on the cell wall surface of various representatives of the fungal kingdom. Titers of the IgG antibodies to Candida albicans mannan are determined in the diagnosis of the candidiasis infection. Owing to high complexity and heterogeneity of the structure of natural mannans, the determination of the exact epitope structure recognized by antibodies is extremely important for the improvement of diagnostic tools based on the detection of anti-mannan antibodies. A high level of the antibodies to mannan was also found in healthy donors. However, it remains undetermined what structural epitope was recognized in this case. The levels of the IgG and IgM antibodies against individual structural fragments of mannan in the blood sera of healthy donors were determined. The anti-mannan antibody response was measured using enzyme immunoassay in a random sample of 31 blood sera of healthy donors on a panel of 11 synthetic mannooligosaccharides of various structures. A high antibody titer was found for the antigens containing the β-mannosidic bond, and the highest values were achieved in the case of two (1→2)-linked β-mannose residues. No significant level of the antibodies was revealed for antigens containing only α-mannose residues. Thus, it can be concluded that β-mannans appear to be much stronger antigens. It is assumed that the detection of antibodies to β-mannans in the sera of healthy donors can be a source of false positive results in the serological diagnosis of the Candida infection.

Cite: Solovev A.S. , Tsarapaev P.V. , Krylov V.B. , Yashunsky D.V. , Kushlinskii N.E. , Nifantiev N.E.
A repertoire of anti-mannan Candida albicans antibodies in the blood sera of healthy donors
Russian Chemical Bulletin. 2023. V.72. N1. P.263-268. DOI: 10.1007/s11172-023-3731-3 WOS Scopus OpenAlex

Identifiers:

≡ Web of science:	WOS:000933481700021
≡ Scopus:	2-s2.0-85148428312
≡ OpenAlex:	W4320736377

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