Structure of the K49 capsular polysaccharide produced by the Acinetobacter baumannii ST10 carriage isolate, NL6

Structure of the K49 capsular polysaccharide produced by the Acinetobacter baumannii ST10 carriage isolate, NL6 Full article

Journal

Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215

Output data

Year: 2025, Volume: 557, Article number : 109646, Pages count : DOI: 10.1016/j.carres.2025.109646

Authors

Kasimova Anastasiya A ¹ , Shneider Mikhail M ² , Shashkov Alexander S ¹ , Kenyon Johanna J ³ , Hall Ruth M ⁴ , Knirel Yuriy A ¹

Affiliations

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
2	M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
3	School of Pharmacy and Medical Sciences, Health Group, Griffith University, Parklands Drive, Gold Coast, Queensland, 4222, Australia; Institute for Biomedicine and Glycomics, Griffith University, Parklands Drive, Gold Coast, Queensland, 4222, Australia. Electronic address: j.kenyon@griffith.edu.au.
4	School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia.

The K49 capsular polysaccharide (CPS) produced by the Acinetobacter baumannii ST10 carriage isolate, NL6, that carries KL49 at the CPS biosynthesis K locus, was studied by sugar analysis along with one- and two-dimensional 1H and 13C NMR spectroscopy. The CPS was found to be comprised of linear trisaccharide units that include 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-d-galacto-non-2-ulosonic acid (5,7-di-N-acetyl-8-epilegionaminic acid; 8eLeg5Ac7Ac), l-FucpNAc and d-GlcpNAc residues. The genetic analysis identified α-d-GlcpNAc as the first sugar, revealing that the 8eLeg5Ac7Ac-(2→3)-α-l-FucpNAc-(1→3)-α-d-GlcpNAc units are linked by WzyKL49 to form an α-GlcpNAc-(1→8)-8eLeg5Ac7Ac linkage. In addition, while KL49 includes two initiating transferase genes, NL6 harbours an ISAba1 interruption in itrB2 indicating that ItrB2, the second initiating transferase that was thought to be redundant, is not needed to make the K49 CPS. The CPS composition was found to be identical to that of a previously reported undefined polysaccharide recovered from A. baumannii ST10 isolate, LAC-4, that has since been shown to carry KL49. Hence, this polysaccharide is also a CPS.

Kasimova A.A. , Shneider M.M. , Shashkov A.S. , Kenyon J.J. , Hall R.M. , Knirel Y.A.
Structure of the K49 capsular polysaccharide produced by the Acinetobacter baumannii ST10 carriage isolate, NL6
Carbohydrate Research. 2025. V.557. 109646 . DOI: 10.1016/j.carres.2025.109646 WOS OpenAlex

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