New Obolenskvirus Phages Brutus and Scipio: Biology, Evolution, and Phage-Host Interaction

New Obolenskvirus Phages Brutus and Scipio: Biology, Evolution, and Phage-Host Interaction Full article

Journal

International Journal of Molecular Sciences
ISSN: 1661-6596 , E-ISSN: 1422-0067

Output data

Year: 2024, Volume: 25, Number: 4, Article number : 2074, Pages count : DOI: 10.3390/ijms25042074

Authors

Evseev Peter V. ^1,2,3 , Shneider Mikhail M. ² , Kolupaeva Lyubov V. ³ , Kasimova Anastasia A. ^4,3 , Timoshina Olga Y. ² , Perepelov Andrey V. ⁴ , Shpirt Anna M. ⁴ , Shelenkov Andrey A. ⁵ , Mikhailova Yulia V. ⁵ , Suzina Natalia E. ⁶ , Knirel Yuriy A. ⁴ , Miroshnikov Konstantin A. ² , Popova Anastasia V. ³

Affiliations

1	Pirogov Russian National Research Medical University, 117997 Moscow, Russia
2	Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
3	State Research Center for Applied Microbiology and Biotechnology, City District Serpukhov, Moscow Region, 142279 Obolensk, Russia
4	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia
5	Central Scientific Research Institute of Epidemiology, 111123 Moscow, Russia
6	Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Center for Biological Research of the Russian Academy of Sciences”, Moscow Region, 142290 Pushchino, Russia

Two novel virulent phages of the genus Obolenskvirus infecting Acinetobacter baumannii, a significant nosocomial pathogen, have been isolated and studied. Phages Brutus and Scipio were able to infect A. baumannii strains belonging to the K116 and K82 capsular types, respectively. The biological properties and genomic organization of the phages were characterized. Comparative genomic, phylogenetic, and pangenomic analyses were performed to investigate the relationship of Brutus and Scipio to other bacterial viruses and to trace the possible origin and evolutionary history of these phages and other representatives of the genus Obolenskvirus. The investigation of enzymatic activity of the tailspike depolymerase encoded in the genome of phage Scipio, the first reported virus infecting A. baumannii of the K82 capsular type, was performed. The study of new representatives of the genus Obolenskvirus and mechanisms of action of depolymerases encoded in their genomes expands knowledge about the diversity of viruses within this taxonomic group and strategies of Obolenskvirus–host bacteria interaction.

Evseev P.V. , Shneider M.M. , Kolupaeva L.V. , Kasimova A.A. , Timoshina O.Y. , Perepelov A.V. , Shpirt A.M. , Shelenkov A.A. , Mikhailova Y.V. , Suzina N.E. , Knirel Y.A. , Miroshnikov K.A. , Popova A.V.
New Obolenskvirus Phages Brutus and Scipio: Biology, Evolution, and Phage-Host Interaction
International Journal of Molecular Sciences. 2024. V.25. N4. 2074 . DOI: 10.3390/ijms25042074 WOS Scopus OpenAlex

Web of science:	WOS:001175125700001
Scopus:	2-s2.0-85185946888
OpenAlex:	W4391653982

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