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Study of Lysozyme Activity in Bird Egg Whites by Fluorescence Polarization Assay Using Chitooligosaccharide Tracer Научная публикация

Журнал Foods
ISSN: 2304-8158
Вых. Данные Год: 2025, Том: 14, Номер: 8, Номер статьи : 1365, Страниц : DOI: 10.3390/foods14081365
Авторы Mukhametova Liliya I. 1 , Zherdev Dmitry O. 1,2 , Kuznetsov Anton N. 3 , Yudina Olga N. 2 , Eremin Sergei A. 1 , Krylov Vadim B. 3 , Nifantiev Nikolay E. 2
Организации
1 Faculty of Chemistry, M.V. Lomonosov Moscow State University, Leninsky Gory 1/3, 119991 Moscow, Russia
2 Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, 119991 Moscow, Russia
3 Laboratory of Synthetic Glycovaccines, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, 119991 Moscow, Russia

Реферат: The storage duration and hatchability of eggs largely depend on the lysozyme content in egg whites; therefore, determining the lysozyme status is important for characterizing their quality. For the first time, a fast and accurate method for determining the active lysozyme in egg whites has been proposed to establish the lysozyme status of eggs using the fluorescence polarization assay and synthetic chitooligosaccharide conjugates with a fluorescent label without sample preparation. The egg whites of hens, black hens, chukars, quails, ducks, geese, turkeys, peacocks, and ostriches were studied. Samples of egg whites from hens, black hens, chukars, and quails demonstrate the possibility of measuring the lysozyme activity. Samples of hen and black hen eggs from a farm showed approximately the same enzymatic activity of lysozyme. A relatively higher enzymatic activity was demonstrated by the samples from quail egg whites; however, a wide range of data was observed among the eggs. Chitooligosaccharide conjugates demonstrate that they bind only to C-type lysozyme, and no interaction with G-type lysozyme has been shown. Lysozyme activity in the egg whites of duck, goose, turkey, peacock, and ostrich eggs has not been detected by using the obtained chitooligosaccharide tracers, which may be related to the structural features of lysozyme in different bird species. Thus, the method of fluorescence polarization (FP), using fluorescently labeled chitopentaoside to determine the lysozyme status, can be used to characterize hen, black hen, chukar, and quail eggs, which will allow for the selection of a batch of eggs with a high content of active lysozyme, for example, for long-term storage.
Библиографическая ссылка: Mukhametova L.I. , Zherdev D.O. , Kuznetsov A.N. , Yudina O.N. , Eremin S.A. , Krylov V.B. , Nifantiev N.E.
Study of Lysozyme Activity in Bird Egg Whites by Fluorescence Polarization Assay Using Chitooligosaccharide Tracer
Foods. 2025. V.14. N8. 1365 . DOI: 10.3390/foods14081365 WOS Scopus OpenAlex
Идентификаторы БД:
≡ Web of science: WOS:001475084000001
≡ Scopus: 2-s2.0-105003547397
≡ OpenAlex: W4409447303
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