The K95 capsular polysaccharide produced by Acinetobacter baumannii isolate MAR18-2212 includes a rarely encountered 3-acetamido-3,6-dideoxy-d-glucose (d-Qui3NAc) sugar

The K95 capsular polysaccharide produced by Acinetobacter baumannii isolate MAR18-2212 includes a rarely encountered 3-acetamido-3,6-dideoxy-d-glucose (d-Qui3NAc) sugar Full article

Journal

Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215

Output data

Year: 2025, Volume: 553, Article number : 109499, Pages count : DOI: 10.1016/j.carres.2025.109499

Authors

Arbatsky Nikolay P ¹ , Shashkov Alexander S ¹ , Sharar Nowshin S ² , Baird Fiona J ³ , Shneider Mikhail M ⁴ , Shpirt Anna M ¹ , Perepelov Andrei V ¹ , Dmitrenok Andrei S ¹ , Mikhailova Yulia V ⁵ , Shelenkov Andrey A ⁵ , Popova Anastasiya V ⁶ , Kenyon Johanna J ⁷ , Knirel Yuriy A ¹

Affiliations

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
2	Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia.
3	School of Pharmacy and Medical Sciences, Health Group, Griffith University, Gold Coast Campus, Southport, Australia.
4	M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
5	Central Research Institute for Epidemiology, Moscow, Russia.
6	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
7	Institute for Biomedicine and Glycomics, Griffith University, Gold Coast Campus, Southport, Australia

The whole genome sequence from Acinetobacter baumannii MAR18-2212, an isolate recovered in 2018 from a human respiratory tract specimen in Russia, was obtained and found to carry KL95 at the capsular polysaccharide (CPS) biosynthesis K locus. KL95 includes a module of four genes (rmlB-rmlA-qdtE-qdtB) predicting synthesis of 3-acetamido-3,6-dideoxy-d-glucose (d-Quip3NAc). The structure of the K95 CPS isolated from MAR18-2212 was established using 1H and 13C NMR spectroscopy, including two-dimensional 1Н,1Н COSY, 1Н,1Н TOCSY, 1Н,1Н ROESY, 1Н,13C HSQC, and 1Н,13C HMBC experiments, and was confirmed by Smith degradation. K95 is composed of branched hexasaccharide K-units containing three d-GalpNAc residues, two d-Galp residues and one residue of d-Quip3NAc. Assignment of the enzymes encoded by KL95 established the role of rmlB-rmlA-qdtE-qdtB in the synthesis of d-Quip3NAc. Five encoded glycosyltransferases and the Wzy polymerase were also assigned to the glycosidic linkages in K95.

Arbatsky N.P. , Shashkov A.S. , Sharar N.S. , Baird F.J. , Shneider M.M. , Shpirt A.M. , Perepelov A.V. , Dmitrenok A.S. , Mikhailova Y.V. , Shelenkov A.A. , Popova A.V. , Kenyon J.J. , Knirel Y.A.
The K95 capsular polysaccharide produced by Acinetobacter baumannii isolate MAR18-2212 includes a rarely encountered 3-acetamido-3,6-dideoxy-d-glucose (d-Qui3NAc) sugar
Carbohydrate Research. 2025. V.553. 109499 . DOI: 10.1016/j.carres.2025.109499 WOS Scopus OpenAlex

Web of science:	WOS:001481464300001
Scopus:	2-s2.0-105003119040
OpenAlex:	W4409534357

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