Structure of K102 Capsular Polysaccharide from Acinetobacter baumannii KZ-1102 and Its Cleavage by Phage Cato Depolymerase

Structure of K102 Capsular Polysaccharide from Acinetobacter baumannii KZ-1102 and Its Cleavage by Phage Cato Depolymerase Full article

Journal

International Journal of Molecular Sciences
ISSN: 1661-6596 , E-ISSN: 1422-0067

Output data

Year: 2025, Volume: 26, Number: 10, Article number : 4727, Pages count : DOI: 10.3390/ijms26104727

Authors

Kasimova Anastasia A. ^1,2 , Arbatsky Nikolay P. ¹ , Gornostal Ekaterina A. ³ , Shneider Mikhail M. ³ , Sheck Eugene A. ⁴ , Shashkov Alexander S. ¹ , Shelenkov Andrey A. ⁵ , Mikhailova Yulia V. ⁵ , Azizov Ilya S. ⁴ , Edelstein Mikhail V. ⁴ , Perepelov Andrey V. ¹ , Shpirt Anna M. ¹ , Miroshnikov Konstantin A. ³ , Popova Anastasia V. ² , Knirel Yuriy A. ¹

Affiliations

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia
2	State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia
3	M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
4	Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, 214019 Smolensk, Russia
5	Central Scientific Research Institute of Epidemiology, 111123 Moscow, Russia

Acinetobacter baumannii is a significant nosocomial pathogen characterized by the ability to produce a wide variety of capsular polysaccharides (CPSs). The structures of a K102-type CPS isolated from A. baumannii KZ-1102 and its Smith degradation product were determined by sugar analysis, 1D and 2D 1H NMR spectroscopy, and 13C NMR spectroscopy. The K102 CPS biosynthesis gene cluster (KL102) contains genes for common sugar synthesis, K unit processing, capsule export, glycosyl transfer, initiating sugar phosphate transfer, and genes that encode d-GlcpNAc/d-GalpNAc dehydrogenase and phosphoglycerol transferase. The CPS is composed of a pentasaccharide repeating unit (K unit) consisting of a tetrasaccharide backbone including one α-d-Galp, three α-d-GlcpNAc residues, and one residue of a β-d-Glcp as a side chain. The tailspike depolymerase of the specific Obolenskvirus phage Cato was found to cleave the α-d-GlcpNAc-(1→6)-α-d-GlcpNAc linkage in the K102 CPS to give the monomer and dimer of the K repeating unit, which were characterized by high-resolution electrospray ionization mass spectrometry as well as 1H and 13C NMR spectroscopy.

Kasimova A.A. , Arbatsky N.P. , Gornostal E.A. , Shneider M.M. , Sheck E.A. , Shashkov A.S. , Shelenkov A.A. , Mikhailova Y.V. , Azizov I.S. , Edelstein M.V. , Perepelov A.V. , Shpirt A.M. , Miroshnikov K.A. , Popova A.V. , Knirel Y.A.
Structure of K102 Capsular Polysaccharide from Acinetobacter baumannii KZ-1102 and Its Cleavage by Phage Cato Depolymerase
International Journal of Molecular Sciences. 2025. V.26. N10. 4727 . DOI: 10.3390/ijms26104727 WOS OpenAlex

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