Carbapenem-resistant Acinetobacter baumannii isolate BAL114 from Vietnam produces capsular polysaccharide with two forms of the K58 unit.

Carbapenem-resistant Acinetobacter baumannii isolate BAL114 from Vietnam produces capsular polysaccharide with two forms of the K58 unit. Full article

Journal

Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215

Output data

Year: 2025, Volume: 556, Article number : 109592, Pages count : DOI: 10.1016/j.carres.2025.109592

Authors

Arbatsky Nikolay P ¹ , Kasimova Anastasiya A ^1,2 , Shashkov Alexander S ¹ , Shneider Mikhail M ³ , Popova Anastasiya V ^4,5 , Hall Ruth M ⁶ , Kenyon Johanna J ^7,8 , Knirel Yuriy A ¹

Affiliations

1	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
2	Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia.
3	M M. Shemyakin & Y A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
4	Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia;
5	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
6	School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia.
7	School of Pharmacy and Medical Sciences, Health Group, Griffith University, Gold Coast Campus, Southport, Australia
8	Institute for Biomedicine and Glycomics, Griffith University, Gold Coast Campus, Southport, Australia

Capsular polysaccharide (CPS) produced by two Acinetobacter baumannii isolates carrying the KL58 locus were recently determined and found to differ due to the presence of additional CPS biosynthesis genes found in prophage. Here, we have examined the CPS produced by a third KL58-carrying A. baumannii isolate, BAL114, which was recovered in 2009 in Vietnam. CPS extracted from BAL114 was studied by sugar analysis, selective solvolysis with trifluoroacetic acid in the presence of 2-methyl-1-propanol, and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy. Consistent with the genetic content of KL58, the polysaccharide was found to include tetrasaccharide K58 units consisting of a 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic (di-N-acetylpseudaminic) acid, d-Gal, d-Glc, and d-GalNAc. However, the linkage between the K58 units was different to the β-d-GalNAc-(1→3)-d-Glc linkage seen previously for other K58 forms. There were two CPS types in the ratio ∼1 : 1 with K58 units linked by either β-(1→3) or β-(1→4) linkages between the d-GalNAc and d-Gal residues.

Arbatsky N.P. , Kasimova A.A. , Shashkov A.S. , Shneider M.M. , Popova A.V. , Hall R.M. , Kenyon J.J. , Knirel Y.A.
Carbapenem-resistant Acinetobacter baumannii isolate BAL114 from Vietnam produces capsular polysaccharide with two forms of the K58 unit.
Carbohydrate Research. 2025. V.556. 109592 . DOI: 10.1016/j.carres.2025.109592 WOS OpenAlex

Web of science:	WOS:001530406000001
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