Increasing the Sensitivity of Aspergillus Galactomannan ELISA Using Silver Nanoparticle-Based Surface-Enhanced Raman Spectroscopy

Increasing the Sensitivity of Aspergillus Galactomannan ELISA Using Silver Nanoparticle-Based Surface-Enhanced Raman Spectroscopy Full article

Journal

Sensors
ISSN: 1424-8220

Output data

Year: 2025, Volume: 25, Number: 14, Article number : 4376, Pages count : DOI: 10.3390/s25144376

Authors

Vasilyeva A.D. ¹ , Yurina L.V. ¹ , Evtushenko E.G. ^2,1 , Gavrilina E.S. ¹ , Krylov V.B. ³ , Nifantiev N.E. ³ , Kurochkin I.N. ^2,1

Affiliations

1	N. M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4 Kosygina Str., 119334 Moscow, Russia
2	Faculty of Chemistry, Lomonosov Moscow State University, 1/3 Leninskie Gory, 119991 Moscow, Russia
3	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia

Galactomannan (GM) is a polysaccharide secreted by opportunistic pathogenic fungi of the Aspergillus genus. It is prescribed as a diagnostic biomarker of invasive aspergillosis in immunocompromised patients by the guidelines for diagnosis and management of Aspergillus diseases. It has been shown previously that the measurement of soluble horseradish peroxidase (HRP) using surface-enhanced Raman scattering (SERS) of 2,3-diaminophenazine enzymatic reaction product on silver nanoparticles is largely superior in detection limit compared to colorimetric readout. In this study, a highly sensitive SERS-based HRP measurement protocol was applied to enzyme-linked immunosorbent assay (ELISA) for GM quantification in biological fluids. The detection limit for GM was 4.3 pg per sample, which is one and a half orders of magnitude lower compared to colorimetric detection with o-phenylenediamine as a substrate and five times more sensitive than ELISA using 3,3′,5,5′-tetramethylbenzidine.

Vasilyeva A.D. , Yurina L.V. , Evtushenko E.G. , Gavrilina E.S. , Krylov V.B. , Nifantiev N.E. , Kurochkin I.N.
Increasing the Sensitivity of Aspergillus Galactomannan ELISA Using Silver Nanoparticle-Based Surface-Enhanced Raman Spectroscopy
Sensors. 2025. V.25. N14. 4376 . DOI: 10.3390/s25144376 WOS Scopus OpenAlex

≡ Web of science:	WOS:001535970300001
≡ Scopus:	2-s2.0-105011672122
≡ OpenAlex:	W4412391359