Chemical Structure of a Branched α-d-Glucan from the Eggs of Sea Urchin Tripneustes gratilla

Chemical Structure of a Branched α-d-Glucan from the Eggs of Sea Urchin Tripneustes gratilla Full article

Journal

International Journal of Molecular Sciences
ISSN: 1661-6596 , E-ISSN: 1422-0067

Output data

Year: 2025, Volume: 26, Number: 21, Article number : 10326, Pages count : DOI: 10.3390/ijms262110326

Authors

Bilan Maria I. ¹ , Argunov Dmitry A. ¹ , Torgov Vladimir I. ¹ , Dmitrenok Andrey S. ¹ , Trung Dinh Thanh ² , Pham Thinh Duc ² , Cao Hang Thi Thuy ² , Usov Anatolii I. ¹ , Nifantiev Nikolay E. ¹

Affiliations

1	The Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, 119991 Moscow, Russia
2	Institute of Oceanography, Vietnam Academy of Science and Technology, 01 Cau Da, Nha Trang 650000, Vietnam

A water-soluble high-molecular neutral polysaccharide (NP) was isolated from the eggs of the sea urchin Tripneustes gratilla. The formation of glucose only upon the treatment of NP by amyloglucosidase and the value of its optical rotation [α]D +233.5 (c 0.2, water) confirmed its belonging to the family of α-d-glucans. According to the results of NMR spectroscopy and methylation analysis, the chains of NP are built up of non-reducing terminal, 4-linked and 4,6-disubstituted glucose residues at a ratio of 1:8:1. A branched structure with an average linear chain length of about five glucose residues was calculated from the spectrum of iodine complex. Contrary to the previously published structure of branched α-d-glucan from the sea urchin Strongylocentrotus nudus bearing single glucose units as branches, the polysaccharide NP contains oligosaccharide branches at position 6, which was confirmed by NMR data. Hence, NP has a glycogen-like structure with a rather high degree of branching, which markedly exceeds that of usual mammalian or fungal glycogens.

Bilan M.I. , Argunov D.A. , Torgov V.I. , Dmitrenok A.S. , Trung D.T. , Pham T.D. , Cao H.T.T. , Usov A.I. , Nifantiev N.E.
Chemical Structure of a Branched α-d-Glucan from the Eggs of Sea Urchin Tripneustes gratilla
International Journal of Molecular Sciences. 2025. V.26. N21. 10326 . DOI: 10.3390/ijms262110326 WOS Scopus OpenAlex

≡ Web of science:	WOS:001612710700001
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