Pleiotrophic Effects of Knockout Mutations in the TPS1 and TPS2 Genes Encoding Trehalose Biosynthesis Enzymes of Saccharomyces cerevisiae

Pleiotrophic Effects of Knockout Mutations in the TPS1 and TPS2 Genes Encoding Trehalose Biosynthesis Enzymes of Saccharomyces cerevisiae Научная публикация

Журнал

Microbiology
ISSN: 0026-2617 , E-ISSN: 1608-3237

Вых. Данные

Год: 2025, Том: 94, Номер: 6, Страницы: 1011-1017 Страниц : 7 DOI: 10.1134/s0026261725600338

Авторы

Kulakovskaya E.V. ¹ , Torgov V.I. ² , Nifantiev N.E. ² , Rekstina V.V. ³ , Kalebina T.S. ³

Организации

1	Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences,” Skryabin Institute of Biochemistry and Physiology of Microorganisms, 142290, Pushchino, Russia
2	Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russia
3	Department of Molecular Biology, Faculty of Biology, Moscow State University, 119234, Moscow, Russia

The TPS1 and TPS2 genes encoding trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase play an important role in protecting yeast and fungal cells from temperature stress, dehydration, and oxidants, since resistance to these stresses requires accumulation of trehalose. In this work, it was shown that Saccharomyces cerevisiae strains with knockout mutations in the TPS1 and TPS2 genes have greater resistance to heavy metal ions and alkaline stress compared to the parental strain. These mutations do not lead to significant changes in the polyphosphate content in the cells, indicating the absence of a direct relationship between phosphorus homeostasis and the ability to accumulate trehalose. In the cells of ∆tps2, the change in localization of the Bgl2p cell wall protein was observed: this protein appeared to be intracellular. It is assumed that the resistance of knockout strains to heavy metal ions and alkali is associated with additional pleiotropic effects of these mutations.

Kulakovskaya E.V. , Torgov V.I. , Nifantiev N.E. , Rekstina V.V. , Kalebina T.S.
Pleiotrophic Effects of Knockout Mutations in the TPS1 and TPS2 Genes Encoding Trehalose Biosynthesis Enzymes of Saccharomyces cerevisiae
Microbiology. 2025. V.94. N6. P.1011-1017. DOI: 10.1134/s0026261725600338 WOS Scopus OpenAlex

Кулаковская Е.В. , Торгов В.И. , Нифантьев Н.Э. , Рекстина В.В. , Калебина Т.С.
ПЛЕЙОТРОПНЫЕ ЭФФЕКТЫ НОКАУТ-МУТАЦИЙ В ГЕНАХ TPS1 И TPS2, КОДИРУЮЩИХ ФЕРМЕНТЫ БИОСИНТЕЗА ТРЕГАЛОЗЫ У SACCHAROMYCES CEREVISIAE
Микробиология. 2025. Т.94. №6. С.573-581. DOI: 10.7868/S3034546425060077 OpenAlex

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≡ Scopus:	2-s2.0-105022053483
≡ OpenAlex:	W7105843322