Protein of Unknown Function from Bacterium Variovorax paradoxus with the Amino Acid Substitution N174k Capable of Forming Schiff Base with a PLP Molecule

Protein of Unknown Function from Bacterium Variovorax paradoxus with the Amino Acid Substitution N174k Capable of Forming Schiff Base with a PLP Molecule Full article

Journal

Crystallography Reports
ISSN: 1063-7745 , E-ISSN: 1562-689X

Output data

Year: 2024, Volume: 69, Number: 6, Pages: 861-865 Pages count : 5 DOI: 10.1134/s1063774524602235

Authors

Ilyasov I.O. ¹ , Minyaev M.E. ² , Rakitina T.V. ³ , Bakunova A.K. ¹ , Popov V.O. ¹ , Bezsudnova E.Yu. ¹ , Boyko K.M. ¹

Affiliations

1	Bach Institute of Biochemistry, Federal Research Centre “Fundamentals of Biotechnology,” Russian Academy of Sciences, 119071, Moscow, Russia
2	Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russia
3	National Research Centre “Kurchatov Institute”, 123098, Moscow, Russia

Pyridoxal 5'-phosphate (PLP)-dependent enzymes are among the most abundant enzymes present in nearly all organisms, where they perform more than 150 different catalytic functions. Based on the three-dimensional structures, these enzymes are classified into seven (I–VII) different fold types. In these enzymes, the cofactor is bound as a Schiff base with the conserved active-site lysine residue. Recently, we have discovered the protein from the bacterium Variovorax paradoxus (VAPA), which belongs to the fold type IV and has a significant structural similarity to transaminases. It contains an asparagine residue at the position of catalytic lysine in fold type IV transaminases and, consequently, it cannot form a Schiff base with PLP and does not have aminotransferase activity. In this study, a single-point mutant of the VAPA protein with the N174K substitution was obtained and its three-dimensional structure was determined. An analysis of the obtained data showed that the introduced mutation restores the ability of VAPA to form a Schiff base with the cofactor.

Ilyasov I.O. , Minyaev M.E. , Rakitina T.V. , Bakunova A.K. , Popov V.O. , Bezsudnova E.Y. , Boyko K.M.
Protein of Unknown Function from Bacterium Variovorax paradoxus with the Amino Acid Substitution N174k Capable of Forming Schiff Base with a PLP Molecule
Crystallography Reports. 2024. V.69. N6. P.861-865. DOI: 10.1134/s1063774524602235 WOS Scopus OpenAlex

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