Loss of a Branch Sugar in the Acinetobacter baumannii K3-Type Capsular Polysaccharide Due To Frameshifts in the gtr6 Glycosyltransferase Gene Leads To Susceptibility To Phage APK37.1

Loss of a Branch Sugar in the Acinetobacter baumannii K3-Type Capsular Polysaccharide Due To Frameshifts in the gtr6 Glycosyltransferase Gene Leads To Susceptibility To Phage APK37.1 Full article

Journal

Microbiology spectrum
ISSN: 2165-0497

Output data

Year: 2023, Volume: 11, Number: 1, DOI: 10.1128/spectrum.03631-22

Authors

Timoshina Olga Y. ¹ , Kasimova Anastasiya A. ² , Shneider Mikhail M. ¹ , Arbatsky Nikolay P. ² , Shashkov Alexander S. ² , Shelenkov Andrey A. ³ , Mikhailova Yuliya V. ³ , Popova Anastasiya V. ⁴ , Hall Ruth M. ⁵ , Knirel Yuriy A. ² , Kenyon Johanna J. ⁶

Affiliations

1	M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
2	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
3	Central Scientific Research Institute of Epidemiology, Moscow, Russia
4	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Russia
5	School of Life and Environmental Science, University of Sydney, Sydney, Australia
6	Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia

The type of capsular polysaccharide (CPS) on the cell surface of Acinetobacter baumannii can determine the specificity of lytic bacteriophage under consideration for therapeutic use. Here, we report the isolation of a phage on an extensively antibiotic resist- ant ST2 A. baumannii isolate AB5001 that carries the KL3 CPS biosynthesis gene cluster pre- dicting a K3-type CPS. As the phage did not infect isolates carrying KL3 or KL22 and known to produce K3 CPS, the structure of the CPS isolated from A. baumannii AB5001 was determined. AB5001 produced a variant CPS form, K3-v1, that lacks the b-D-GlcpNAc side chain attached to the D-Galp residue in the K3 structure. Inspection of the KL3 sequence in the genomes of AB5001 and other phage-susceptible isolates with a KL3 locus revealed single-base deletions in gtr6, causing loss of the Gtr6 glycosyltransferase that adds the missing D-GlcpNAc side chain to the K3 CPS. Hence, the presence of this sugar pro- foundly restricts the ability of the phage to digest the CPS. The 41-kb linear double- stranded DNA (dsDNA) phage genome was identical to the genome of a phage isolated on a K37-producing isolate and thus was named APK37.1. APK37.1 also infected isolates carrying KL116. Consistent with this, K3-v1 resembles the K37 and K116 structures. APK37.1 is a Friunavirus belonging to the Autographiviridae family. The phage-encoded tail spike depolymerase DpoAPK37.1 was not closely related to Dpo encoded by other sequenced Friunaviruses, including APK37 and APK116.

Timoshina O.Y. , Kasimova A.A. , Shneider M.M. , Arbatsky N.P. , Shashkov A.S. , Shelenkov A.A. , Mikhailova Y.V. , Popova A.V. , Hall R.M. , Knirel Y.A. , Kenyon J.J.
Loss of a Branch Sugar in the Acinetobacter baumannii K3-Type Capsular Polysaccharide Due To Frameshifts in the gtr6 Glycosyltransferase Gene Leads To Susceptibility To Phage APK37.1
Microbiology spectrum. 2023. V.11. N1. DOI: 10.1128/spectrum.03631-22 WOS Scopus OpenAlex

Web of science:	WOS:000916185000001
Scopus:	2-s2.0-85148114310
OpenAlex:	W4317213728

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OpenAlex	14
Scopus	10
Web of science	12