Sciact
  • EN
  • RU

Lipopolysaccharide of Pantoea agglomerans 7460: O-specific polysaccharide and lipid A structures and biological activity Full article

Journal Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215
Output data Year: 2020, Volume: 496, Article number : 108132, Pages count : DOI: 10.1016/j.carres.2020.108132
Authors Zdorovenko Evelina L. 1 , Kadykova Alexandra A. 1 , Shashkov Alexander S. 1 , Varbanets Liudmyla D. 2 , Bulyhina Tetiana V. 2 , Knirel Yuriy A. 1
Affiliations
1 N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991, Moscow, Russia
2 D.K. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences, 154 Zabolotnoho Str., 03143, Kiev, Ukraine

Abstract: Lipopolysaccharide (LPS) was isolated from Pantoea agglomerans 7460 cells by phenol-water extraction. Mild acid degradation allowed to separate OPS and lipid A. Lipid A was analyzed by negative-ion mode ESI MS and found to consist mainly of hexaacylated derivative containing biphosphorylated GlcN disaccharide, four 14:0 (3-OH), 18:0 and 12:0 fatty acids. The structure of the O-specific polysaccharide was established by chemical, NMR and computational methods:Image 2 The LPS of Р. agglomerans 7460 showed low level of toxicity and pyrogenicity to compare with LPS of E. coli O55:B5 and pyrogenal, respectively. The ability of the modified (succinylated) LPS, which have lost its toxicity, to block the toxic effects of native LPS has been shown.
Cite: Zdorovenko E.L. , Kadykova A.A. , Shashkov A.S. , Varbanets L.D. , Bulyhina T.V. , Knirel Y.A.
Lipopolysaccharide of Pantoea agglomerans 7460: O-specific polysaccharide and lipid A structures and biological activity
Carbohydrate Research. 2020. V.496. 108132 . DOI: 10.1016/j.carres.2020.108132 WOS Scopus OpenAlex
Identifiers:
Web of science: WOS:000571539000001
Scopus: 2-s2.0-85089810583
OpenAlex: W3049309219
Citing:
DB Citing
OpenAlex 4
Scopus 3
Web of science 3
Altmetrics: