Labeling of Polysaccharides with Biotin and Fluorescent Dyes | Sciact

Labeling of Polysaccharides with Biotin and Fluorescent Dyes Научная публикация

Журнал

Polysaccharides
ISSN: 2673-4176

Вых. Данные

Год: 2024, Том: 5, Номер: 1, Страницы: 1-15 Страниц : 15 DOI: 10.3390/polysaccharides5010001

Авторы

Tuzikov Alexander ¹ , Shilova Nadezhda ¹ , Ovchinnikova Tatiana ¹ , Nokel Alexey ¹ , Patova Olga ² , Knirel Yuriy ³ , Chernova Tatiana ⁴ , Gorshkova Tatiana ^2,4 , Bovin Nicolai ¹

Организации

1	M. M. Shemyakin–Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997 Moscow, Russia
2	Institute of Physiology of Federal Research Centre “Komi Science Centre of the Urals Branch of the Russian Academy of Sciences”, 167982 Syktyvkar, Russia
3	Zelinsky Institute of Organic Chemistry RAS, 47 Leninsky Prosp, 119991 Moscow, Russia
4	Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center of the Russian Academy of Sciences, Lobachevsky str., 2/31, 420111 Kazan, Russia

Examples of labeling polysaccharides at hydroxyl groups are described in this paper, which are especially in demand for molecules with a blocked reducing end. The protocols presented are suitable for the microscale synthesis of labeled polysaccharides that do not require a chromatography step for isolation. Examples of hydroxyl labeling include (1) direct modification with fluorescein isothiocyanate; (2) reaction with a fluorescein-dichlorotriazine derivative; (3) reaction with biotin-dichlorotriazine; (4) indirect two-step modification (given for glycosphingolipid) with glutaric anhydride followed by amidation with aminospacered BODIPY or SuCy5. The labeling of carboxyl groups of hyaluronic acid with BODIPY is also described. The staining of plant tissue sections with biotinylated polysaccharide versus being fluorescein labeled is compared.

Tuzikov A. , Shilova N. , Ovchinnikova T. , Nokel A. , Patova O. , Knirel Y. , Chernova T. , Gorshkova T. , Bovin N.
Labeling of Polysaccharides with Biotin and Fluorescent Dyes
Polysaccharides. 2024. V.5. N1. P.1-15. DOI: 10.3390/polysaccharides5010001 OpenAlex

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W4390274880

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