Structure elucidation and gene cluster annotation of the O-antigen of Halomonas titanicae TAT1 containing three residues of 2,3-diacetamido-2,3-dideoxy-D-glucuronic acid

Structure elucidation and gene cluster annotation of the O-antigen of Halomonas titanicae TAT1 containing three residues of 2,3-diacetamido-2,3-dideoxy-D-glucuronic acid Full article

Journal

Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215

Output data

Year: 2022, Volume: 521, Article number : 108650, Pages count : DOI: 10.1016/j.carres.2022.108650

Authors

Perepelov Andrei V. ¹ , Sokolova Diyana S. ² , Tourova Tatiana P. ² , Shashkov Alexander S. ¹ , Kasimova Anastasiya A. ¹ , Nazina Tamara N. ²

Affiliations

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 119991, Russia
2	Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, 119071, Russian Federation

A halotolerant hydrocarbon-oxidizing bacterium Halomonas titanicae strain TAT1 was isolated from a petroleum reservoir. The O-polysaccharide (O-antigen) was isolated from the lipopolysaccharide of H. titanicae TAT1 and studied by component analyses and 1D and 2D NMR spectroscopy. The following structure of the repeating linear pentasaccharide O-unit, containing only aminosugars, was established: →4)-β-d-GlcpNAc3NAcA-(1 → 4)-β-d-GlcpNAc3NAcA-(1 → 6)-α-d-GlcpNAc-(1 → 4)-β-d-GlcpNAc3NAcA-(1 → 6)-α-d-GlcpNAc-(→, where d-GlcNAc3NAcA indicates 2,3-diacetamido-2,3-dideoxy-d-glucuronic acid. The O-antigen gene cluster was identified in the genome of H. titanicae TAT1 and compared with available database sequences. The genes revealed in the O-antigen gene cluster and the assigned functions of putative proteins were consistent with the established polysaccharide structure.

Perepelov A.V. , Sokolova D.S. , Tourova T.P. , Shashkov A.S. , Kasimova A.A. , Nazina T.N.
Structure elucidation and gene cluster annotation of the O-antigen of Halomonas titanicae TAT1 containing three residues of 2,3-diacetamido-2,3-dideoxy-D-glucuronic acid
Carbohydrate Research. 2022. V.521. 108650 . DOI: 10.1016/j.carres.2022.108650 WOS Scopus OpenAlex

Web of science:	WOS:000862886200004
Scopus:	2-s2.0-85136108900
OpenAlex:	W4292166098

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