Identification of a new DC-SIGN binding pentamannoside epitope within the complex structure of Candida albicans mannan

Identification of a new DC-SIGN binding pentamannoside epitope within the complex structure of Candida albicans mannan Full article

Journal

Cell Surface
ISSN: 2468-2330

Output data

Year: 2023, Volume: 10, Article number : 100109, Pages count : DOI: 10.1016/j.tcsw.2023.100109

Authors

Krylov Vadim B. ¹ , Gómez-Redondo Marcos ² , Solovev Arsenii S. ¹ , Yashunsky Dmitry V. ¹ , Brown Alistair J.P. ³ , Stappers Mark H.T. ³ , Gow Neil A.R. ³ , Ardá Ana ² , Jiménez-Barbero Jesús ^2,4,5,6 , Nifantiev Nikolay E. ¹

Affiliations

1	Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
2	CIC bioGUNE, Basque Research Technology Alliance, BRTA, 48160 Derio, Spain
3	Medical Research Council Centre for Medical Mycology, University of Exeter, Exeter EX4 4QD, United Kingdom
4	Centro de Investigacion Biomedica En Red de Enfermedades Respiratorias, Madrid, Spain
5	Department of Organic & Inorganic Chemistry, Faculty of Science and Technology, University of the Basque Country, 48940 Leioa, Spain
6	IKERBASQUE, Basque Foundation for Science and Technology, Euskadi Plaza 5, 48009 Bilbao, Spain

The dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) is an innate immune C-type lectin receptor that recognizes carbohydrate-based pathogen associated with molecular patterns of various bacteria, fungi, viruses and protozoa. Although a range of highly mannosylated glycoproteins have been shown to induce signaling via DC-SIGN, precise structure of the recognized oligosaccharide epitope is still unclear. Using the array of oligosaccharides related to selected fragments of main fungal antigenic polysaccharides we revealed a highly specific pentamannoside ligand of DC-SIGN, consisting of α-(1 → 2)-linked mannose chains with one inner α-(1 → 3)-linked unit. This structural motif is present in Candida albicans cell wall mannan and corresponds to its antigenic factors 4 and 13b. This epitope is not ubiquitous in other yeast species and may account for the species-specific nature of fungal recognition via DC-SIGN. The discovered highly specific oligosaccharide ligands of DC-SIGN are tractable tools for interdisciplinary investigations of mechanisms of fungal innate immunity and anti-Candida defense. Ligand- and receptor-based NMR data demonstrated the pentasaccharide-to-DC-SIGN interaction in solution and enabled the deciphering of the interaction topology.

Krylov V.B. , Gómez-Redondo M. , Solovev A.S. , Yashunsky D.V. , Brown A.J.P. , Stappers M.H.T. , Gow N.A.R. , Ardá A. , Jiménez-Barbero J. , Nifantiev N.E.
Identification of a new DC-SIGN binding pentamannoside epitope within the complex structure of Candida albicans mannan
Cell Surface. 2023. V.10. 100109 . DOI: 10.1016/j.tcsw.2023.100109 Scopus OpenAlex

≡ Scopus:	2-s2.0-85165600608
≡ OpenAlex:	W4385143915