Structure, Physicochemical Properties and Biological Activity of Lipopolysaccharide from the Rhizospheric Bacterium Ochrobactrum quorumnocens T1Kr02, Containing d-Fucose Residues

Structure, Physicochemical Properties and Biological Activity of Lipopolysaccharide from the Rhizospheric Bacterium Ochrobactrum quorumnocens T1Kr02, Containing d-Fucose Residues Full article

Journal

International Journal of Molecular Sciences
ISSN: 1661-6596 , E-ISSN: 1422-0067

Output data

Year: 2024, Volume: 25, Number: 4, Article number : 1970, Pages count : DOI: 10.3390/ijms25041970

Authors

Krivoruchko Aleksandra A. ¹ , Zdorovenko Evelina L. ² , Ivanova Maria F. ³ , Kostina Ekaterina E. ³ , Fedonenko Yulia P. ^4,5 , Shashkov Alexander S. ² , Dmitrenok Andrey S. ² , Ul’chenko Elizaveta A. ⁶ , Tkachenko Oksana V. ³ , Astankova Anastasia S. ¹ , Burygin Gennady L. ^1,3,5

Affiliations

1	Department of Organic and Bioorganic Chemistry, Institute of Chemistry, Saratov State University, 410012 Saratov, Russia
2	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia
3	Department of Plant Breeding, Selection, and Genetics, Faculty of Agronomy, Saratov State University of Genetics, Biotechnology and Engineering Named after N.I. Vavilov, 410012 Saratov, Russia
4	Department of Biochemistry and Biophysics, Faculty of Biology, Saratov State University, 410012 Saratov, Russia
5	Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences, 410049 Saratov, Russia
6	Department of Biomedical Products, Faculty of Chemical Pharmaceutical Technologies, D.I. Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russia

Lipopolysaccharides (LPSs) are major components of the outer membranes of Gram-negative bacteria. In this work, the structure of the O-polysaccharide of Ochrobactrum quorumnocens T1Kr02 was identified by nuclear magnetic resonance (NMR), and the physical–chemical properties and biological activity of LPS were also investigated. The NMR analysis showed that the O-polysaccharide has the following structure: →2)-β-d-Fucf-(1→3)-β-d-Fucp-(1→. The structure of the periplasmic glucan coextracted with LPS was established by NMR spectroscopy and chemical methods: →2)-β-d-Glcp-(1→. Non-stoichiometric modifications were identified in both polysaccharides: 50% of d-fucofuranose residues at position 3 were O-acetylated, and 15% of d-Glcp residues at position 6 were linked with succinate. This is the first report of a polysaccharide containing both d-fucopyranose and d-fucofuranose residues. The fatty acid analysis of the LPS showed the prevalence of 3-hydroxytetradecanoic, hexadecenoic, octadecenoic, lactobacillic, and 27-hydroxyoctacosanoic acids. The dynamic light scattering demonstrated that LPS (in an aqueous solution) formed supramolecular particles with a size of 72.2 nm and a zeta-potential of –21.5 mV. The LPS solution (10 mkg/mL) promoted the growth of potato microplants under in vitro conditions. Thus, LPS of O. quorumnocens T1Kr02 can be recommended as a promoter for plants and as a source of biotechnological production of d-fucose.

Krivoruchko A.A. , Zdorovenko E.L. , Ivanova M.F. , Kostina E.E. , Fedonenko Y.P. , Shashkov A.S. , Dmitrenok A.S. , Ul’chenko E.A. , Tkachenko O.V. , Astankova A.S. , Burygin G.L.
Structure, Physicochemical Properties and Biological Activity of Lipopolysaccharide from the Rhizospheric Bacterium Ochrobactrum quorumnocens T1Kr02, Containing d-Fucose Residues
International Journal of Molecular Sciences. 2024. V.25. N4. 1970 . DOI: 10.3390/ijms25041970 WOS Scopus OpenAlex

Web of science:	WOS:001173060100001
Scopus:	2-s2.0-85185972868
OpenAlex:	W4391576075

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