Pectobacterium versatile Bacteriophage Possum: A Complex Polysaccharide-Deacetylating Tail Fiber as a Tool for Host Recognition in Pectobacterial Schitoviridae

Pectobacterium versatile Bacteriophage Possum: A Complex Polysaccharide-Deacetylating Tail Fiber as a Tool for Host Recognition in Pectobacterial Schitoviridae Full article

Journal

International Journal of Molecular Sciences
ISSN: 1661-6596 , E-ISSN: 1422-0067

Output data

Year: 2022, Volume: 23, Number: 19, Article number : 11043, Pages count : DOI: 10.3390/ijms231911043

Authors

Lukianova Anna A. ^1,2 , Evseev Peter V. ¹ , Shneider Mikhail M. ¹ , Dvoryakova Elena A. ^3,1 , Tokmakova Anna D. ^1,4,2 , Shpirt Anna M. ⁵ , Kabilov Marsel R. ⁶ , Obraztsova Ekaterina A. ¹ , Shashkov Alexander S. ⁵ , Ignatov Alexander N. ^7,2 , Knirel Yuriy A. ⁵ , Dzhalilov Fevzi S.-U. ² , Miroshnikov Konstantin A. ^1,2

Affiliations

1	Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
2	Department of Plant Protection, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, 127134 Moscow, Russia
3	Biological Faculty, Department of Microbiology, Lomonosov Moscow State University, 119991 Moscow, Russia
4	School of Biological and Medical Physics, Moscow Institute of Physics and Technology (MIPT), 141701 Dolgoprudny, Russia
5	Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia
6	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia
7	Agrarian and Technological Institute, RUDN University, 117198 Moscow, Russia

Novel, closely related phages Possum and Horatius infect Pectobacterium versatile, a phytopathogen causing soft rot in potatoes and other essential plants. Their properties and genomic composition define them as N4-like bacteriophages of the genus Cbunavirus, a part of a recently formed family Schitoviridae. It is proposed that the adsorption apparatus of these phages consists of tail fibers connected to the virion through an adapter protein. Tail fibers possess an enzymatic domain. Phage Possum uses it to deacetylate O-polysaccharide on the surface of the host strain to provide viral attachment. Such an infection mechanism is supposed to be common for all Cbunavirus phages and this feature should be considered when designing cocktails for phage control of soft rot.

Lukianova A.A. , Evseev P.V. , Shneider M.M. , Dvoryakova E.A. , Tokmakova A.D. , Shpirt A.M. , Kabilov M.R. , Obraztsova E.A. , Shashkov A.S. , Ignatov A.N. , Knirel Y.A. , Dzhalilov F.S.-U. , Miroshnikov K.A.
Pectobacterium versatile Bacteriophage Possum: A Complex Polysaccharide-Deacetylating Tail Fiber as a Tool for Host Recognition in Pectobacterial Schitoviridae
International Journal of Molecular Sciences. 2022. V.23. N19. 11043 . DOI: 10.3390/ijms231911043 WOS Scopus OpenAlex

Web of science:	WOS:000867778100001
Scopus:	2-s2.0-85139930266
OpenAlex:	W4296481846

DB	Citing
OpenAlex	11
Scopus	8
Web of science	8