“3-in-1” Hybrid Biocatalysts: Association of Yeast Cells Immobilized in a Sol–Gel Matrix for Determining Sewage Pollution

“3-in-1” Hybrid Biocatalysts: Association of Yeast Cells Immobilized in a Sol–Gel Matrix for Determining Sewage Pollution Full article

Journal

ACS Applied Materials & Interfaces
ISSN: 1944-8244 , E-ISSN: 1944-8252

Output data

Year: 2023, Volume: 15, Number: 40, Pages: 47779-47789 Pages count : 11 DOI: 10.1021/acsami.3c09897

Authors

Kamanina Olga A. ¹ , Lantsova Elizaveta A. ¹ , Rybochkin Pavel V. ¹ , Arlyapov Vyacheslav A. ¹ , Saverina Evgeniya A. ^1,2 , Kulikovskaya Natalia S. ² , Perepukhov Alexander M. ² , Vereshchagin Anatoly N. ² , Ananikov Valentine P. ²

Affiliations

1	Tula State University, pr. Lenina 92, 300012 Tula, Russia
2	Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 119991 Moscow, Russia

This study presents a novel ″3-in-1″ hybrid biocatalyst design that combines the individual efficiency of microorganisms while avoiding negative interactions between them. Yeast cells of Ogataea polymorpha VKM Y-2559, Blastobotrys adeninivorans VKM Y-2677, and Debaryomyces hansenii VKM Y-2482 were immobilized in an organosilicon material by using the sol–gel method, resulting in a hybrid biocatalyst. The catalytic activity of the immobilized microorganism mixture was evaluated by employing it as the bioreceptor element of a biosensor. Optical and scanning electron microscopies were used to examine the morphology of the biohybrid material. Elemental distribution analysis confirmed the encapsulation of yeast cells in a matrix composed of methyltriethoxysilane (MTES) and tetraethoxysilane (TEOS) (85 and 15 vol %, respectively). The resulting heterogeneous biocatalyst exhibited excellent performance in determining the biochemical oxygen demand (BOD) index in real surface water samples, with a sensitivity coefficient of 50 ± 3 × 10–3·min–1, a concentration range of 0.3–31 mg/L, long-term stability for 25 days, and a relative standard deviation of 3.8%. These findings demonstrate the potential of the developed hybrid biocatalyst for effective pollution monitoring and wastewater treatment applications.

Kamanina O.A. , Lantsova E.A. , Rybochkin P.V. , Arlyapov V.A. , Saverina E.A. , Kulikovskaya N.S. , Perepukhov A.M. , Vereshchagin A.N. , Ananikov V.P.
“3-in-1” Hybrid Biocatalysts: Association of Yeast Cells Immobilized in a Sol–Gel Matrix for Determining Sewage Pollution
ACS Applied Materials & Interfaces. 2023. V.15. N40. P.47779-47789. DOI: 10.1021/acsami.3c09897 WOS Scopus OpenAlex

Web of science:	WOS:001076213700001
Scopus:	2-s2.0-85175587357
OpenAlex:	W4387241741

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OpenAlex	6
Scopus	4