Affinity characteristics of anti-β-(1→3)-d-glucan monoclonal antibody 3G11 by fluorescence polarization immunoassay

Affinity characteristics of anti-β-(1→3)-d-glucan monoclonal antibody 3G11 by fluorescence polarization immunoassay Full article

Journal

Russian Chemical Bulletin
ISSN: 1573-9171 , E-ISSN: 1066-5285

Output data

Year: 2021, Volume: 70, Number: 5, Pages: 975-981 Pages count : 7 DOI: 10.1007/s11172-021-3175-6

Authors

Mukhametova L.I. ¹ , Krylov V.B. ² , Solovev A.S. ^1,2 , Yashunsky D.V. ² , Matveev A.L. ³ , Tikunova N.V. ³ , Eremin S.A. ¹ , Nifantiev N.E. ²

Affiliations

1	Lomonosov Moscow State University , Moscow , Russian Federation
2	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow (Russian Federation)
3	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation;

β-(1→3)-D-Glucan is a branched polysaccharide present in the inner layer of the cell wall of various representatives of the fungal kingdom. Therefore, this polysaccharide is considered as a common marker for the laboratory diagnosis of mycoses of various etiologies. However, low concentration of β-(1→3)-D-glucan in biological fluids (∼40 pg mL−1) requires the development of highly sensitive methods for its determination. One of the most convenient types of bioassays is based on immunochemical approaches, in which the most important stage is selection and characterization of monoclonal antibodies (mAb). In this communication, the binding constant of mAb 3G11 and the synthetic oligosaccharide β-D-Glcp-[(1→3)-Glcp]8, which is related to the linear moiety of fungal glucan, was determined in solution for the first time using fluorescence polarization immunoassay (FPIA) method. The obtained information will be used subsequently for the development of analytical systems for FPIA detection of β-(1→3)-D-glucan.

Mukhametova L.I. , Krylov V.B. , Solovev A.S. , Yashunsky D.V. , Matveev A.L. , Tikunova N.V. , Eremin S.A. , Nifantiev N.E.
Affinity characteristics of anti-β-(1→3)-d-glucan monoclonal antibody 3G11 by fluorescence polarization immunoassay
Russian Chemical Bulletin. 2021. V.70. N5. P.975-981. DOI: 10.1007/s11172-021-3175-6 WOS Scopus OpenAlex

≡ Web of science:	WOS:000657311100023
≡ Scopus:	2-s2.0-85107041994
≡ OpenAlex:	W3168193573