Acinetobacter baumannii K116 capsular polysaccharide structure is a hybrid of the K14 and revised K37 structures

Acinetobacter baumannii K116 capsular polysaccharide structure is a hybrid of the K14 and revised K37 structures Full article

Journal

Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215

Output data

Year: 2019, Volume: 484, Article number : 107774, Pages count : DOI: 10.1016/j.carres.2019.107774

Authors

Shashkov Alexander S. ¹ , Cahill Sarah M. ² , Arbatsky Nikolay P. ¹ , Westacott Amy C. ² , Kasimova Anastasiya A. ^3,1 , Shneider Mikhail M. ^4,5 , Popova Anastasiya V. ^5,6,7 , Shagin Dmitry A. ⁸ , Shelenkov Andrey A. ⁸ , Mikhailova Yuliya V. ⁸ , Yanushevich Yurii G. ⁸ , Edelstein Mikhail V. ⁵ , Kenyon Johanna J. ² , Knirel Yuriy A. ¹

Affiliations

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
2	Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Queensland University of Technology, Brisbane, Australia
3	Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia
4	M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
5	Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russia
6	Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia
7	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia
8	Central Scientific Research Institute of Epidemiology, Moscow, Russia

The genome of Acinetobacter baumannii clinical isolate, MAR-303, recovered in Russia was sequenced and found to contain a novel gene cluster at the A. baumannii K locus for capsule biosynthesis. The gene cluster, designated KL116, included four genes for glycosyltransferases (Gtrs) and a gene for a Wzy polymerase responsible for joining oligosaccharide K units into the capsular polysaccharide (CPS). The arrangement of KL116 was a hybrid of previously described A. baumannii gene clusters, with two gtr genes and the wzy gene shared by KL37 and the two other gtr genes found in KL14. The structure of the K116 CPS was established by sugar analysis and Smith degradation, along with one- and two-dimensional 1H and 13C NMR spectroscopy. The CPS is composed of branched pentasaccharide K units containing only neutral sugars, with three monosaccharides in the main chain and a disaccharide side chain. The K116 unit shares internal sugar linkages with the K14 and K37 units, corresponding to the presence of shared gtr genes in the gene clusters. However, the specific linkage formed by Wzy was discrepant between K116 and the previously reported K37 CPS produced by A. baumannii isolate NIPH146. The K37 structure was therefore revised in this study, and the corrected Wzy linkage found to be identical to the Wzy linkage in K116. The KL116, KL14 and KL37 gene clusters were found in genomes of a variety of A. baumannii strain backgrounds, indicating their global distribution.

Shashkov A.S. , Cahill S.M. , Arbatsky N.P. , Westacott A.C. , Kasimova A.A. , Shneider M.M. , Popova A.V. , Shagin D.A. , Shelenkov A.A. , Mikhailova Y.V. , Yanushevich Y.G. , Edelstein M.V. , Kenyon J.J. , Knirel Y.A.
Acinetobacter baumannii K116 capsular polysaccharide structure is a hybrid of the K14 and revised K37 structures
Carbohydrate Research. 2019. V.484. 107774 . DOI: 10.1016/j.carres.2019.107774 WOS Scopus OpenAlex

Web of science:	WOS:000487202700005
Scopus:	2-s2.0-85070564421
OpenAlex:	W2967347137

DB	Citing
OpenAlex	29
Scopus	25