Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster

Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster Full article

Journal

International Journal of Biological Macromolecules
ISSN: 1879-0003 , E-ISSN: 0141-8130

Output data

Year: 2019, Volume: 128, Pages: 101-106 Pages count : 6 DOI: 10.1016/j.ijbiomac.2019.01.080

Authors

Kenyon Johanna J. ¹ , Arbatsky Nikolay P. ² , Sweeney Emma L. ¹ , Shashkov Alexander S. ² , Shneider Mikhail M. ^3,4 , Popova Anastasia V. ^4,5,6 , Hall Ruth M. ⁷ , Knirel Yuriy A. ²

Affiliations

1	Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia
2	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
3	M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
4	Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russia
5	Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia
6	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia
7	School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia

A new capsular polysaccharide (CPS) biosynthesis gene cluster, KL16, was found in the genome sequence of a clinical Acinetobacter baumannii ST25 isolate, D4. The variable part of KL16 contains a module of genes for synthesis of 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid (5,7-di-N-acetylpseudaminic acid, Pse5Ac7Ac), a gene encoding ItrA3 that initiates the CPS synthesis with d-GlcpNAc, and two glycosyltransferase (Gtr) genes. The K16 CPS was studied by sugar analysis and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy, and shown to be built up of linear trisaccharide repeats containing d-galactose (d-Gal), N-acetyl-d-glucosamine (d-GlcNAc), and Pse5Ac7Ac. The d-Galp residue is linked to the d-GlcpNAc initiating sugar via a β-(1 → 3) linkage evidently formed by a Gtr5 variant, Gtr5K16, encoded in KL16. This reveals an altered or relaxed substrate specificity of this variant as the majority of Gtr5-type glycosyltransferases have previously been shown to form a β-d-Galp-(1 → 3)-d-GalpNAc linkage. The β-Psep5Ac7Ac-(2 → 4)-d-Galp linkage is predicted to be formed by the other glycosyltransferase, Gtr37, which does not match members of any known glycosyltransferase family.

Kenyon J.J. , Arbatsky N.P. , Sweeney E.L. , Shashkov A.S. , Shneider M.M. , Popova A.V. , Hall R.M. , Knirel Y.A.
Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster
International Journal of Biological Macromolecules. 2019. V.128. P.101-106. DOI: 10.1016/j.ijbiomac.2019.01.080 WOS Scopus OpenAlex

Web of science:	WOS:000463305100014
Scopus:	2-s2.0-85060464491
OpenAlex:	W2909616554

DB	Citing
OpenAlex	26
Scopus	23
Web of science	21