The K46 and K5 capsular polysaccharides produced by Acinetobacter baumannii NIPH 329 and SDF have related structures and the side-chain non-ulosonic acids are 4-O-acetylated by phage-encoded O-acetyltransferases

The K46 and K5 capsular polysaccharides produced by Acinetobacter baumannii NIPH 329 and SDF have related structures and the side-chain non-ulosonic acids are 4-O-acetylated by phage-encoded O-acetyltransferases Full article

Journal

PLOS ONE
ISSN: 1932-6203

Output data

Year: 2019, Volume: 14, Number: 6, Article number : e0218461, Pages count : DOI: 10.1371/journal.pone.0218461

Authors

Kenyon Johanna J ¹ , Arbatsky Nikolay P ² , Shneider Mikhail M ³ , Popova Anastasiya V ^4,5 , Dmitrenok Andrei S ² , Kasimova Anastasiya A ^6,2 , Shashkov Alexander S ² , Hall Ruth M ⁷ , Knirel Yuriy A ²

Affiliations

1	Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia
2	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
3	M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
4	Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia
5	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia
6	Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia
7	School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia

Acinetobacter baumannii isolate NIPH 329 carries a novel capsular polysaccharide (CPS) gene cluster, designated KL46, that is closely related to the KL5 locus in A. baumannii isolate SDF but includes genes for synthesis of 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic (di-N-acetylpseudaminic) acid (Pse5Ac7Ac) instead of the corresponding D-glycero-D-galacto isomer (di-N-acetyllegionaminic acid) (Leg5Ac7Ac). In agreement with the genetic content of KL46, chemical studies of the K46 CPS produced by NIPH 329 revealed a branched tetrasaccharide repeat (K unit) with an overall structure the same as K5 from SDF but with â-Pse5Ac7Ac replacing α-Leg5Ac7Ac. As for K5, the K46 unit begins with d-GalpNAc and includes α-d-GlcpNAc-(1→3)-d-GalpNAc and α-d-Galp-(1→6)-d-GlcpNAc linkages, formed by Gtr14 and Gtr15 glycosyltransferases, respectively. The Gtr94K46 glycosyltransferase, which is related to Gtr13K5, links Pse5Ac7Ac to d-Galp in the growing K unit via a â-(2→6) linkage. Nearly identical Wzy enzymes connect the K46 and K5 units via a α-D-GalpNAc-(1→3)-α-D-Galp linkage to form closely related CPSs. Both Pse5Ac7Ac in K46 and Leg5Ac7Ac in K5 are acetylated at O4 but no acetyltransferase gene is present in KL46 or KL5. Related acetyltransferases were found encoded in the NIPH 329 and SDF genomes, but not in other strains carrying an unacetylated Pse or Leg derivative in the CPS. The genes encoding the acetyltransferases were in different putative phage genomes. However, related acetyltransferases were rare among the >3000 publically available genome sequences.

Kenyon J.J. , Arbatsky N.P. , Shneider M.M. , Popova A.V. , Dmitrenok A.S. , Kasimova A.A. , Shashkov A.S. , Hall R.M. , Knirel Y.A.
The K46 and K5 capsular polysaccharides produced by Acinetobacter baumannii NIPH 329 and SDF have related structures and the side-chain non-ulosonic acids are 4-O-acetylated by phage-encoded O-acetyltransferases
PLOS ONE. 2019. V.14. N6. e0218461 . DOI: 10.1371/journal.pone.0218461 WOS Scopus OpenAlex

Web of science:	WOS:000484893500052
Scopus:	2-s2.0-85067443715
OpenAlex:	W2949463068

DB	Citing
OpenAlex	31
Scopus	24
Web of science	25