Abstract: In this report, we describe the fluorescent labeling of bacterial polysaccharides (Escherichia coli O86:B7, Escherichia coli O19ab, Pseudomonas aeruginosa O10a10b, and Shigella flexneri 2b) at the “natural” amino group of their phosphoethanolamine moiety. Two protocols for labeling are compared: 1) on a scale of a few mg of the polysaccharide, with a dialysis procedure for purification from excessive reagents; and 2) on a scale of 0.1 mg of the polysaccharide, with a simple precipitation procedure instead of dialysis. The microscale version is sufficient for comfortable cytofluorometric analysis. The resulting probes were found to specifically bind to human dendritic cells in a dose-dependent manner. The used limited set of polysaccharides did not allow us even to get close to understanding which dendritic cell-associated lectins and which cognate polysaccharide epitopes are involved in recognition, but the proposed microscale protocol allows to generate a library of fluorescent probes for further mapping of the polysaccharide specificity of the dendritic cells.

Cite: Tuzikov A.B. , Rapoport E.M. , Khaidukov S.V. , Nokel E.A. , Knirel Y.A. , Bovin N.V.
Synthesis of bodipy-labeled bacterial polysaccharides and their interaction with human dendritic cells
Glycoconjugate Journal. 2021. V.38. N3. P.369-374. DOI: 10.1007/s10719-021-09993-9 WOS Scopus OpenAlex

Identifiers:

Web of science:	WOS:000635030500001
Scopus:	2-s2.0-85103413406
OpenAlex:	W3141853750

Citing:

DB	Citing
OpenAlex	4
Scopus	3
Web of science	3

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