Electroactive Biofilms of Activated Sludge Microorganisms on a Nanostructured Surface as the Basis for a Highly Sensitive Biochemical Oxygen Demand Biosensor

Electroactive Biofilms of Activated Sludge Microorganisms on a Nanostructured Surface as the Basis for a Highly Sensitive Biochemical Oxygen Demand Biosensor Full article

Journal

Sensors
ISSN: 1424-8220

Output data

Year: 2022, Volume: 22, Number: 16, Article number : 6049, Pages count : DOI: 10.3390/s22166049

Authors

Kurbanalieva Saniyat ¹ , Arlyapov Vyacheslav ¹ , Kharkova Anna ¹ , Perchikov Roman ¹ , Kamanina Olga ¹ , Melnikov Pavel ² , Popova Nadezhda ³ , Machulin Andrey ⁴ , Tarasov Sergey ⁴ , Saverina Evgeniya ⁵ , Vereshchagin Anatoly ⁵ , Reshetilov Anatoly ⁴

Affiliations

1	Laboratory of Biologically Active Compounds and Biocomposites, Tula State University, Lenin Pr. 92, Tula 300012, Russia
2	M. V. Lomonosov Institute of Fine Chemical Technologies, MIREA—Russian Technological University, Prosp. Vernadskogo 86, Moscow 119571, Russia
3	Federal State Budgetary Institution of Science Institute of Physical Chemistry and Electrochemistry of the Russian Academy of Sciences, Leninsky Prosp., 31 k. 4., Moscow 119071, Russia
4	Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences—A Separate Subdivision of the FRC Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Prosp. Science 3, Pushchino 142290, Russia
5	N. D. Zelinsky Institute of Organic Chemistry, Leninsky Pr. 47, Moscow 119991, Russia

The possibility of the developing a biochemical oxygen demand (BOD) biosensor based on electroactive biofilms of activated sludge grown on the surface of a graphite-paste electrode modified with carbon nanotubes was studied. A complex of microscopic methods controlled biofilm formation: optical microscopy with phase contrast, scanning electron microscopy, and laser confocal microscopy. The features of charge transfer in the obtained electroactive biofilms were studied using the methods of cyclic voltammetry and electrochemical impedance spectroscopy. The rate constant of the interaction of microorganisms with the extracellular electron carrier (0.79 ± 0.03 dm3(g s)−1) and the heterogeneous rate constant of electron transfer (0.34 ± 0.02 cm s−1) were determined using the cyclic voltammetry method. These results revealed that the modification of the carbon nanotubes’ (CNT) electrode surface makes it possible to create electroactive biofilms. An analysis of the metrological and analytical characteristics of the created biosensors showed that the lower limit of the biosensor based on an electroactive biofilm of activated sludge is 0.41 mgO2/dm3, which makes it possible to analyze almost any water sample. Analysis of 12 surface water samples showed a high correlation (R2 = 0.99) with the results of the standard method for determining biochemical oxygen demand.

Kurbanalieva S. , Arlyapov V. , Kharkova A. , Perchikov R. , Kamanina O. , Melnikov P. , Popova N. , Machulin A. , Tarasov S. , Saverina E. , Vereshchagin A. , Reshetilov A.
Electroactive Biofilms of Activated Sludge Microorganisms on a Nanostructured Surface as the Basis for a Highly Sensitive Biochemical Oxygen Demand Biosensor
Sensors. 2022. V.22. N16. 6049 . DOI: 10.3390/s22166049 WOS Scopus OpenAlex

Web of science:	WOS:000845468300001
Scopus:	2-s2.0-85136692925
OpenAlex:	W4291825455

DB	Citing
OpenAlex	19
Scopus	16
Web of science	17