Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial Host

Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial Host Full article

Journal

Frontiers in Microbiology
ISSN: 1664-302X

Output data

Year: 2019, Volume: 9, Article number : 430806, Pages count : DOI: 10.3389/fmicb.2018.03288

Authors

Kabanova Anastasia P ^1,2 , Shneider Mikhail M ² , Korzhenkov Aleksei A ³ , Bugaeva Eugenia N ² , Miroshnikov Kirill K ⁴ , Zdorovenko Evelina L ⁵ , Kulikov Eugene E ⁴ , Toschakov Stepan V ^3,4 , Ignatov Alexander N ² , Knirel Yuriy A ⁵ , Miroshnikov Konstantin A ^1,2

Affiliations

1	Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
2	Research Center “PhytoEngineering” Ltd., Rogachevo, Russia
3	Immanuel Kant Baltic Federal University, Kaliningrad, Russia
4	Winogradsky Institute of Microbiology, Federal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, Moscow, Russia
5	Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia

Dickeya solani is a recently emerged virulent bacterial potato pathogen that poses a major threat to world agriculture. Because of increasing antibiotic resistance and growing limitations in antibiotic use, alternative antibacterials such as bacteriophages are being developed. Myoviridae bacteriophages recently re-ranked as a separate Ackermannviridae family, such as phage PP35 described in this work, are the attractive candidates for this bacterial biocontrol. PP35 has a very specific host range due to the presence of tail spike protein PP35 gp156, which can depolymerize the O-polysaccharide (OPS) of D. solani. The D. solani OPS structure, →2)-β-D-6-deoxy-D-altrose-(1→, is so far unique among soft-rot Pectobacteriaceae, though it may exist in non-virulent environmental Enterobacteriaceae. The phage tail spike depolymerase degrades the shielding polysaccharide, and launches the cell infection process. We hypothesize that non-pathogenic commensal bacteria may maintain the population of the phage in soil environment.

Kabanova A.P. , Shneider M.M. , Korzhenkov A.A. , Bugaeva E.N. , Miroshnikov K.K. , Zdorovenko E.L. , Kulikov E.E. , Toschakov S.V. , Ignatov A.N. , Knirel Y.A. , Miroshnikov K.A.
Host Specificity of the Dickeya Bacteriophage PP35 Is Directed by a Tail Spike Interaction With Bacterial O-Antigen, Enabling the Infection of Alternative Non-pathogenic Bacterial Host
Frontiers in Microbiology. 2019. V.9. 430806 . DOI: 10.3389/fmicb.2018.03288 WOS Scopus OpenAlex

Web of science:	WOS:000455534400001
Scopus:	2-s2.0-85064401714
OpenAlex:	W2908607797

DB	Citing
OpenAlex	24
Scopus	21
Web of science	20