The KL24 gene cluster and a genomic island encoding a Wzy polymerase contribute genes needed for synthesis of the K24 capsular polysaccharide by the multiply antibiotic resistant Acinetobacter baumannii isolate RCH51

The KL24 gene cluster and a genomic island encoding a Wzy polymerase contribute genes needed for synthesis of the K24 capsular polysaccharide by the multiply antibiotic resistant Acinetobacter baumannii isolate RCH51 Full article

Journal

Microbiology (UK)
ISSN: 1350-0872 , E-ISSN: 1465-2080

Output data

Year: 2017, Volume: 163, Number: 3, Pages: 355-363 Pages count : 9 DOI: 10.1099/mic.0.000430

Authors

Kenyon Johanna J ^1,2 , Kasimova Anastasiya A ^3,4 , Shneider Mikhail M ⁵ , Shashkov Alexander S ⁴ , Arbatsky Nikolay P ⁴ , Popova Anastasiya V ^6,7 , Miroshnikov Konstantin A ⁵ , Hall Ruth M ⁸ , Knirel Yuriy A ⁴

Affiliations

1	Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia
2	School of Molecular Bioscience, The University of Sydney, Sydney, Australia
3	Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia
4	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
5	M.M. Shemyakin and Y.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences,Moscow,Russia
6	Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia
7	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia
8	1School of Molecular Bioscience, The University of Sydney, Sydney, Australia

The whole-genome sequence of the multiply antibiotic resistant Acinetobacter baumannii isolate RCH51 belonging to sequence type ST103 (Institut Pasteur scheme) revealed that the set of genes at the capsule locus, KL24, includes four genes predicted to direct the synthesis of 3-acetamido-3,6-dideoxy-d-galactose (d-Fuc3NAc), and this sugar was found in the capsular polysaccharide (CPS). One of these genes, fdtE, encodes a novel bifunctional protein with an N-terminal FdtA 3,4-ketoisomerase domain and a C-terminal acetyltransferase domain. KL24 lacks a gene encoding a Wzy polymerase to link the oligosaccharide K units to form the CPS found associated with isolate RCH51, and a wzy gene was found in a small genomic island (GI) near the cpn60 gene. This GI is in precisely the same location as another GI carrying wzy and atr genes recently found in several A. baumannii isolates, but it does not otherwise resemble it. The CPS isolated from RCH51, studied by sugar analysis and 1D and 2D 1H and 13C NMR spectroscopy, revealed that the K unit has a branched pentasaccharide structure made up of Gal, GalNAc and GlcNAc residues with d-Fuc3NAc as a side branch, and the K units are linked via a β-d-GlcpNAc-(1→3)-β-d-Galp linkage formed by the Wzy encoded by the GI. The functions of the glycosyltransferases encoded by KL24 were assigned to formation of specific bonds. A correspondence between the order of the genes in KL24 and other KL and the order of the linkages they form was noted, and this may be useful in future predictions of glycosyltransferase specificities.

Kenyon J.J. , Kasimova A.A. , Shneider M.M. , Shashkov A.S. , Arbatsky N.P. , Popova A.V. , Miroshnikov K.A. , Hall R.M. , Knirel Y.A.
The KL24 gene cluster and a genomic island encoding a Wzy polymerase contribute genes needed for synthesis of the K24 capsular polysaccharide by the multiply antibiotic resistant Acinetobacter baumannii isolate RCH51
Microbiology (UK). 2017. V.163. N3. P.355-363. DOI: 10.1099/mic.0.000430 WOS Scopus OpenAlex

Web of science:	WOS:000400276500008
Scopus:	2-s2.0-85017033872
OpenAlex:	W2581071874

DB	Citing
OpenAlex	30
Scopus	27
Web of science	27