Lysozyme's lectin-like characteristics facilitates its immune defense function

Lysozyme's lectin-like characteristics facilitates its immune defense function Научная публикация

Журнал

Quarterly Reviews of Biophysics
ISSN: 1469-8994 , E-ISSN: 0033-5835

Вых. Данные

Год: 2017, Том: 50, Номер статьи : e9, Страниц : DOI: 10.1017/s0033583517000075

Авторы

Zhang Ruiyan ^1,2 , Wu Lisha ³ , Eckert Thomas ⁴ , Burg-Roderfeld Monika ⁵ , Rojas-Macias Miguel A. ⁵ , Lütteke Thomas ⁵ , Krylov Vadim B. ⁶ , Argunov Dmitry A. ⁶ , Datta Aritreyee ⁷ , Markart Philipp ^8,9 , Guenther Andreas ^10,11 , Norden Bengt ³ , Schauer Roland ⁸ , Bhunia Anirban ⁷ , Enani Mushira Abdelaziz ¹² , Billeter Martin ¹³ , Scheidig Axel J. ¹ , Nifantiev Nikolay E. ⁶ , Siebert Hans-Christian ²

Организации

1	Department of Structural Biology, Institute of Zoology, Christian-Albrechts-University, Am Botanischen Garten 1-9, 24118 Kiel, Germany
2	RI-B-NT Research Institute of Bioinformatics and Nanotechnology, Franziusallee 177, 24148 Kiel, Germany
3	Department of Chemical and Biological Engineering, Chalmers University of Technology, 41296 Gothenburg, Sweden
4	Clinic of Obstetrics, Gynecology and Andrology for Small and Large Animals, Justus-Liebig-University, Justus-Liebig-University Giessen, Frankfurter Str. 106, 35392 Giessen, Germany
5	Institute for Veterinary Physiology and Biochemistry, Justus-Liebig-University, Frankfurter Str.100, 35392 Giessen, Germany
6	Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky prospect 47, 119991 Moscow, Russian Federation
7	Department of Biophysics, Bose Institute, P-1/12 CIT Scheme VII (M), Kolkata 700054, India
8	Institute of Biochemistry, Christian-Albrechts-University, Olshausenstrasse 40, 24098 Kiel, Germany
9	Pneumology, Heart-Thorax-Center Fulda, Pacelliallee 4 – 36043 Fulda, Germany
10	Medical Clinic II, Justus-Liebig-University, Klinikstraße 33, 35392 Giessen, Germany
11	Member of the German Center for Lung Research (DZL)
12	Infectious Diseases Division, Department of Medicine, King Fahad Medical City, PO Box 59046, Riyadh 11525, Kingdom of Saudi Arabia
13	Department of Chemistry and Molecular Biology, University of Gothenburg, 40530 Gothenburg, Sweden

Interactions between human lysozyme (HL) and the lipopolysaccharide (LPS) of Klebsiella pneumoniae O1, a causative agent of lung infection, were identified by surface plasmon resonance. To characterize the molecular mechanism of this interaction, HL binding to synthetic disaccharides and tetrasaccharides representing one and two repeating units, respectively, of the O-chain of this LPS were studied. pH-dependent structural rearrangements of HL after interaction with the disaccharide were observed through nuclear magnetic resonance. The crystal structure of the HL-tetrasaccharide complex revealed carbohydrate chain packing into the A, B, C, and D binding sites of HL, which primarily occurred through residue-specific, direct or water-mediated hydrogen bonds and hydrophobic contacts. Overall, these results support a crucial role of the Glu35/Asp53/Trp63/Asp102 residues in HL binding to the tetrasaccharide. These observations suggest an unknown glycan-guided mechanism that underlies recognition of the bacterial cell wall by lysozyme and may complement the HL immune defense function.

Zhang R. , Wu L. , Eckert T. , Burg-Roderfeld M. , Rojas-Macias M.A. , Lütteke T. , Krylov V.B. , Argunov D.A. , Datta A. , Markart P. , Guenther A. , Norden B. , Schauer R. , Bhunia A. , Enani M.A. , Billeter M. , Scheidig A.J. , Nifantiev N.E. , Siebert H-C.
Lysozyme's lectin-like characteristics facilitates its immune defense function
Quarterly Reviews of Biophysics. 2017. V.50. e9 . DOI: 10.1017/s0033583517000075 WOS Scopus OpenAlex

≡ Web of science:	WOS:000402401200001
≡ Scopus:	2-s2.0-85041004776
≡ OpenAlex:	W2622366444