Structure and gene cluster of the K93 capsular polysaccharide of Acinetobacter baumannii B11911 containing 5-N-Acetyl-7-N-[(R)-3-hydroxybutanoyl]pseudaminic acid

Structure and gene cluster of the K93 capsular polysaccharide of Acinetobacter baumannii B11911 containing 5-N-Acetyl-7-N-[(R)-3-hydroxybutanoyl]pseudaminic acid Full article

Journal

Biokhimiya
ISSN: 0006-2979 , E-ISSN: 1608-3040

Output data

Year: 2017, Volume: 82, Number: 4, Pages: 483-489 Pages count : 7 DOI: 10.1134/s0006297917040101

Authors

Kasimova A A ¹ , Shneider M M ² , Arbatsky N P ¹ , Popova A V ³ , Shashkov A S ¹ , Miroshnikov K A ² , Balaji Veeraraghavan ⁴ , Biswas Indranil ⁵ , Knirel Yu A ¹

Affiliations

1	Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russia
2	Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 119997, Moscow, Russia
3	Moscow Institute of Physics and Technology, 141701, Dolgoprudny, Moscow Region, Russia
4	Department of Clinical Microbiology, Christian Medical College, Vellore, 632004, Tamil Nadu, India
5	Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS, 66160, USA

Capsular polysaccharide (CPS) assigned to the K93 type was isolated from the bacterium Acinetobacter baumannii B11911 and studied by sugar analysis along with oneand two-dimensional 1H and 13C NMR spectroscopy. The CPS was found to contain a derivative of pseudaminic acid, and the structure of the branched tetrasaccharide repeating unit was established. Genes in the KL93 capsule biosynthesis locus were annotated and found to be consistent with the CPS structure established. The K93 CPS has the α-D-Galp-(1→6)-β-D-Galp-(1→3)-D-GalpNAc trisaccharide fragment in common with the K14 CPS of Acinetobacter nosocomialis LUH 5541 and A. baumannii D46. It also shares the β-D-Galp-(1→3)-DGalpNAc disaccharide fragment and the corresponding predicted Gal transferase Gtr5, as well as the initiating GalNAc-1P transferase ItrA2, with a number of A. baumannii strains.

Kasimova A.A. , Shneider M.M. , Arbatsky N.P. , Popova A.V. , Shashkov A.S. , Miroshnikov K.A. , Balaji V. , Biswas I. , Knirel Y.A.
Structure and gene cluster of the K93 capsular polysaccharide of Acinetobacter baumannii B11911 containing 5-N-Acetyl-7-N-[(R)-3-hydroxybutanoyl]pseudaminic acid
Biokhimiya. 2017. V.82. N4. P.483-489. DOI: 10.1134/s0006297917040101 WOS Scopus OpenAlex

Web of science:	WOS:000399430800010
Scopus:	2-s2.0-85017513953
OpenAlex:	W2607457966

DB	Citing
OpenAlex	29
Scopus	25
Web of science	26