Capsule-Targeting Depolymerases Derived from Acinetobacter baumannii Prophage Regions

Capsule-Targeting Depolymerases Derived from Acinetobacter baumannii Prophage Regions Full article

Journal

International Journal of Molecular Sciences
ISSN: 1661-6596 , E-ISSN: 1422-0067

Output data

Year: 2022, Volume: 23, Number: 9, Article number : 4971, Pages count : DOI: 10.3390/ijms23094971

Authors

Drobiazko Alena Y. ^1,2 , Kasimova Аnastasia A. ³ , Evseev Peter V. ⁴ , Shneider Mikhail M. ⁴ , Klimuk Evgeniy I. ⁵ , Shashkov Alexander S. ³ , Dmitrenok Andrei S. ³ , Chizhov Alexander O. ³ , Slukin Pavel V. ⁶ , Skryabin Yuriy P. ⁶ , Volozhantsev Nikolay V. ⁶ , Miroshnikov Konstantin A. ⁴ , Knirel Yuriy A. ³ , Popova Anastasia V. ⁶

Affiliations

1	Center of Life Sciences, Skolkovo Institute of Science and Technology, 121205 Skolkovo, Russia
2	Moscow Institute of Physics and Technology (National Research University), 141700 Dolgoprudny, Russia
3	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia
4	Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
5	Institute of Molecular Genetics of National Research Centre «Kurchatov Institute», 123182 Moscow, Russia
6	State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia

In this study, several different depolymerases encoded in the prophage regions of Acinetobacter baumannii genomes have been bioinformatically predicted and recombinantly produced. The identified depolymerases possessed multi-domain structures and were identical or closely homologous to various proteins encoded in other A. baumannii genomes. This means that prophage-derived depolymerases are widespread, and different bacterial genomes can be the source of proteins with polysaccharide-degrading activities. For two depolymerases, the specificity to capsular polysaccharides (CPSs) of A. baumannii belonging to K1 and K92 capsular types (K types) was determined. The data obtained showed that the prophage-derived depolymerases were glycosidases that cleaved the A. baumannii CPSs by the hydrolytic mechanism to yield monomers and oligomers of the K units. The recombinant proteins with established enzymatic activity significantly reduced the mortality of Galleria mellonella larvae infected with A. baumannii of K1 and K92 capsular types. Therefore, these enzymes can be considered as suitable candidates for the development of new antibacterials against corresponding A. baumannii K types.

Drobiazko A.Y. , Kasimova А.A. , Evseev P.V. , Shneider M.M. , Klimuk E.I. , Shashkov A.S. , Dmitrenok A.S. , Chizhov A.O. , Slukin P.V. , Skryabin Y.P. , Volozhantsev N.V. , Miroshnikov K.A. , Knirel Y.A. , Popova A.V.
Capsule-Targeting Depolymerases Derived from Acinetobacter baumannii Prophage Regions
International Journal of Molecular Sciences. 2022. V.23. N9. 4971 . DOI: 10.3390/ijms23094971 WOS Scopus OpenAlex

Web of science:	WOS:000796099000001
Scopus:	2-s2.0-85128999566
OpenAlex:	W4225134871

DB	Citing
OpenAlex	14
Scopus	13
Web of science	11