Structure of the K128 capsular polysaccharide produced by Acinetobacter baumannii KZ-1093 from Kazakhstan

Structure of the K128 capsular polysaccharide produced by Acinetobacter baumannii KZ-1093 from Kazakhstan Full article

Journal

Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215

Output data

Year: 2019, Volume: 485, Article number : 107814, Pages count : DOI: 10.1016/j.carres.2019.107814

Authors

Arbatsky Nikolay P. ¹ , Kasimova Anastasiya A. ^2,1 , Shashkov Alexander S. ¹ , Shneider Mikhail M. ^3,4 , Popova Anastasiya V. ^4,5,6 , Shagin Dmitry A. ⁷ , Shelenkov Andrey A. ⁷ , Mikhailova Yuliya V. ⁷ , Yanushevich Yurii G. ⁷ , Azizov Ilya S. ⁴ , Edelstein Mikhail V. ⁴ , Hall Ruth M. ⁸ , Kenyon Johanna J. ⁹ , Knirel Yuriy A. ¹

Affiliations

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
2	Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia
3	M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
4	Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russia
5	Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia
6	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia
7	Central Scientific Research Institute of Epidemiology, Moscow, Russia
8	School of Life and Environmental Sciences, University of Sydney, Sydney, Australia
9	Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Queensland University of Technology, Brisbane, Australia

The structure of the K128 capsular polysaccharide (CPS) produced by Acinetobacter baumannii isolate KZ-1093 from Kazakhstan was established by sugar analysis and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy. The CPS was found to consist of branched pentasaccharide repeating units containing only neutral sugars, and its composition and topology are closely related to those of the A. baumannii K116 CPS. The K128 and K116 oligosaccharide units differ in the linkage between the disaccharide side chain and the main chain, with a β-(1 → 6) linkage in K128 replacing a β-(1 → 4) linkage in K116. The linkages between the repeating units in the K128 and K116 CPSs are also different, with K128 units linked by β-d-GalpNAc-(1 → 4)-d-Galp, and β-d-GalpNAc-(1 → 3)-d-Galp linkages between K116 units. The KZ-1093 genome was sequenced and the CPS biosynthesis gene cluster at the chromosomal K locus was designated KL128. Consistent with the CPS structures, KL128 differs from KL116 in one glycosyltransferase gene and the gene for the Wzy polymerase. In KL128, the gtr200 glycosyltransferase gene replaces gtr76 in KL116, and Gtr200 was therefore assigned to the different β-d-GalpNAc-(1 → 6)-d-Galp linkage in K128. Similarly, the WzyK128 polymerase could be assigned to the β-d-GalpNAc-(1 → 4)-d-Galp linkage between the K128 units.

Arbatsky N.P. , Kasimova A.A. , Shashkov A.S. , Shneider M.M. , Popova A.V. , Shagin D.A. , Shelenkov A.A. , Mikhailova Y.V. , Yanushevich Y.G. , Azizov I.S. , Edelstein M.V. , Hall R.M. , Kenyon J.J. , Knirel Y.A.
Structure of the K128 capsular polysaccharide produced by Acinetobacter baumannii KZ-1093 from Kazakhstan
Carbohydrate Research. 2019. V.485. 107814 . DOI: 10.1016/j.carres.2019.107814 WOS Scopus OpenAlex

Web of science:	WOS:000491973000003
Scopus:	2-s2.0-85072191561
OpenAlex:	W2972472110

DB	Citing
OpenAlex	15
Scopus	13