Fucose-Rich Sulfated Polysaccharides from Two Vietnamese Sea Cucumbers Bohadschia argus and Holothuria (Theelothuria) spinifera: Structures and Anticoagulant Activity

Fucose-Rich Sulfated Polysaccharides from Two Vietnamese Sea Cucumbers Bohadschia argus and Holothuria (Theelothuria) spinifera: Structures and Anticoagulant Activity Научная публикация

Журнал

Marine Drugs
ISSN: 1660-3397

Вых. Данные

Год: 2022, Том: 20, Номер: 6, Номер статьи : 380, Страниц : DOI: 10.3390/md20060380

Авторы

Ustyuzhanina Nadezhda E. ¹ , Bilan Maria I. ¹ , Dmitrenok Andrey S. ¹ , Tsvetkova Eugenia A. ¹ , Nikogosova Sofya P. ¹ , Hang Cao Thi Thuy ² , Thinh Pham Duc ² , Trung Dinh Thanh ² , Van Tran Thi Thanh ² , Shashkov Alexander S. ¹ , Usov Anatolii I. ¹ , Nifantiev Nikolay E. ¹

Организации

1	The Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, 119991 Moscow, Russia
2	Chemical Analysis and Technology Development Department, NhaTrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, 02 Hung Vuong Street, Nhatrang 650000, Vietnam

Fucosylated chondroitin sulfates (FCSs) FCS-BA and FCS-HS, as well as fucan sulfates (FSs) FS-BA-AT and FS-HS-AT were isolated from the sea cucumbers Bohadschia argus and Holothuria (Theelothuria) spinifera, respectively. Purification of the polysaccharides was carried out by anion-exchange chromatography on DEAE-Sephacel column. Structural characterization of polysaccharides was performed in terms of monosaccharide and sulfate content, as well as using a series of non-destructive NMR spectroscopic methods. Both FCSs were shown to contain a chondroitin core [→3)-β-d-GalNAc-(1→4)-β-d-GlcA-(1→]n bearing sulfated fucosyl branches at O-3 of every GlcA residue in the chain. These fucosyl residues were different in pattern of sulfation: FCS-BA contained Fuc2S4S, Fuc3S4S and Fuc4S at a ratio of 1:8:2, while FCS-HS contained these residues at a ratio of 2:2:1. Polysaccharides differed also in content of GalNAc4S6S and GalNAc4S units, the ratios being 14:1 for FCS-BA and 4:1 for FCS-HS. Both FCSs demonstrated significant anticoagulant activity in clotting time assay and potentiated inhibition of thrombin, but not of factor Xa. FS-BA-AT was shown to be a regular linear polymer of 4-linked α-L-fucopyranose 3-sulfate, the structure being confirmed by NMR spectra of desulfated polysaccharide. In spite of considerable sulfate content, FS-BA-AT was practically devoid of anticoagulant activity. FS-HS-AT cannot be purified completely from contamination of some FCS. Its structure was tentatively represented as a mixture of chains identical with FS-BA-AT and other chains built up of randomly sulfated alternating 4- and 3-linked α-L-fucopyranose residues.

Ustyuzhanina N.E. , Bilan M.I. , Dmitrenok A.S. , Tsvetkova E.A. , Nikogosova S.P. , Hang C.T.T. , Thinh P.D. , Trung D.T. , Van T.T.T. , Shashkov A.S. , Usov A.I. , Nifantiev N.E.
Fucose-Rich Sulfated Polysaccharides from Two Vietnamese Sea Cucumbers Bohadschia argus and Holothuria (Theelothuria) spinifera: Structures and Anticoagulant Activity
Marine Drugs. 2022. V.20. N6. 380 . DOI: 10.3390/md20060380 WOS Scopus OpenAlex

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