The K5 capsular polysaccharide of the bacterium Acinetobacter baumannii SDF with the same K unit containing Leg5Ac7Ac as the K7 capsular polysaccharide but a different linkage between the K units

The K5 capsular polysaccharide of the bacterium Acinetobacter baumannii SDF with the same K unit containing Leg5Ac7Ac as the K7 capsular polysaccharide but a different linkage between the K units Full article

Journal

Russian Chemical Bulletin
ISSN: 1573-9171 , E-ISSN: 1066-5285

Output data

Year: 2019, Volume: 68, Number: 1, Pages: 163-167 Pages count : 5 DOI: 10.1007/s11172-019-2432-4

Authors

Arbatsky N.P. ¹ , Kenyon J.J. ² , Shashkov A.S. ¹ , Shneider M.M. ³ , Popova A.V. ^4,5 , Kalinchuk N.A. ¹ , Hall R.M. ⁶ , Knirel Yu.A. ¹

Affiliations

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47 Leninsky prosp., 119991 Moscow, Russian Federation
2	Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia
3	M. M. Shemyakin—Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 ul. Miklukho-Maklaya, 117997 Moscow, Russian Federation
4	Moscow Institute of Physics and Technology, 9 Institutskii per., 141701 Dolgoprudny, Moscow Region, Russian Federation
5	State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Moscow Region, Russian Federation
6	School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia

The K5 capsular polysaccharide (CPS) was isolated from the bacterium Acinetobacter baumannii (A. baumannii) SDF and studied by 1D and 2D 1H and 13C NMR spectroscopy before and after O-deacetylation and partial acid hydrolysis. The CPS was found to be composed of branched tetrasaccharide repeats (K units) containing 5,7-diacetamido-3,5,7,9-tetradeoxy-D-glycero-D-galacto-non-2-ulosonic acid (Leg5Ac7Ac). The KL5 capsule biosynthesis gene cluster at the K locus is consistent with the composition and structure of the CPS. KL5 is almost identical to the KL7 gene ulosonic acid (Leg5Ac7Ac A. baumannii LUH5533 that has been characterized earlier, and differs only in the gene for Wzy polymerase that is responsible for the formation of the linkage between the K units. The K5 CPS from strain SDF and the K7 CPS from strain LUH5533 have the same K-unit structure but a different linkage between the K units, which is formed by distinct Wzy polymerases. As opposite to Leg5Ac7Ac in the K7 CPS, this monosaccharide is O-acetylated at position 4 in the K5 CPS. No acetyltransferase for this modification of the K5 CPS is present in the KL5 gene cluster, and hence it is encoded by a gene located elsewhere in the genome.

Arbatsky N.P. , Kenyon J.J. , Shashkov A.S. , Shneider M.M. , Popova A.V. , Kalinchuk N.A. , Hall R.M. , Knirel Y.A.
The K5 capsular polysaccharide of the bacterium Acinetobacter baumannii SDF with the same K unit containing Leg5Ac7Ac as the K7 capsular polysaccharide but a different linkage between the K units
Russian Chemical Bulletin. 2019. V.68. N1. P.163-167. DOI: 10.1007/s11172-019-2432-4 WOS Scopus OpenAlex

Web of science:	WOS:000464854400025
Scopus:	2-s2.0-85064347709
OpenAlex:	W2938278088

DB	Citing
OpenAlex	16
Scopus	14
Web of science	13