Structure and gene cluster of the O antigen of Escherichia coli F17, a candidate for a new O-serogroup

Structure and gene cluster of the O antigen of Escherichia coli F17, a candidate for a new O-serogroup Научная публикация

Журнал

International Journal of Biological Macromolecules
ISSN: 1879-0003 , E-ISSN: 0141-8130

Вых. Данные

Год: 2019, Том: 124, Страницы: 389-395 Страниц : 7 DOI: 10.1016/j.ijbiomac.2018.11.149

Авторы

Knirel Yuriy A. ¹ , Ivanov Pavel A. ² , Senchenkova Sofiya N. ¹ , Naumenko Olesya I. ^3,1 , Ovchinnikova Olga O. ¹ , Shashkov Alexander S. ¹ , Golomidova Alla K. ² , Babenko Vladislav V. ⁴ , Kulikov Eugene E. ^5,2 , Letarov Andrey V. ^6,5

Организации

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
2	S. N. Winogradsky Institute of Microbiology, Federal Research Centre “Fundamentals of Biotechnology”, Russian Academy of Sciences, Moscow, Russian Federation
3	Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russian Federation
4	Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, Moscow, Russian Federation
5	Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russian Federation
6	Faculty of Biology, Lomonosov Moscow State University, Moscow, Russian Federation

Escherichia coli F17 isolated from horse feces was studied in respect to the O antigen (O polysaccharide) structure and genetics. The lipopolysaccharide was isolated by phenol-water extraction of bacterial cells and cleaved by mild acid hydrolysis to yield the O polysaccharide, which was studied by sugar analysis and selective solvolysis with CF3CO2H along with one- and two-dimensional 1H and 13C NMR spectroscopy. The O polysaccharide was found to have a branched pentasaccharide repeat (O-unit) containing one residue each of d-galactose, d-mannose, l-rhamnose, d-glucuronic acid, and N-acetyl-d-glucosamine; about 2/3 units bear a side-chain glucose residue. To our knowledge, the F17 O-polysaccharide structure established is unique among known bacterial polysaccharide structures. The O-antigen gene cluster of E. coli F17 between the conserved genes galF and gnd was sequenced and found to be 99% identical to that of E. coli 102,755 assigned to a novel OgN8 genotype (A. Iguchi, S. Iyoda, K. Seto, H. Nishii, M. Ohnishi, H. Mekata, Y. Ogura, T. Hayashi, Front. Microbiol. 7 (2016) 765). Genes in the cluster were annotated taking into account the F17 O-polysaccharide structure. The data obtained confirm that E. coli F17 and E. coli strains belonging to the OgN8 genotype can be considered as a candidate to a new E. coli O-serogroup. The O antigen of this novel type was demonstrated to make for an effective shield protecting the intimate outer membrane surface of bacteria from direct interaction with bacteriophages.

Knirel Y.A. , Ivanov P.A. , Senchenkova S.N. , Naumenko O.I. , Ovchinnikova O.O. , Shashkov A.S. , Golomidova A.K. , Babenko V.V. , Kulikov E.E. , Letarov A.V.
Structure and gene cluster of the O antigen of Escherichia coli F17, a candidate for a new O-serogroup
International Journal of Biological Macromolecules. 2019. V.124. P.389-395. DOI: 10.1016/j.ijbiomac.2018.11.149 WOS Scopus OpenAlex

Web of science:	WOS:000456899300039
Scopus:	2-s2.0-85057818831
OpenAlex:	W2901159114

БД	Цитирований
OpenAlex	15
Scopus	12
Web of science	12