Human antibodies eluted from ligand-free Sepharose capable of binding bacterial polysaccharides and sulfated glycans

Human antibodies eluted from ligand-free Sepharose capable of binding bacterial polysaccharides and sulfated glycans Full article

Journal

Molecular Immunology
ISSN: 0161-5890 , E-ISSN: 1872-9142

Output data

Year: 2019, Volume: 106, Pages: 63-68 Pages count : 6 DOI: 10.1016/j.molimm.2018.12.011

Authors

Dobrochaeva K.L. ¹ , Khasbiullina N.R. ^3,1 , Shilova N.V. ¹ , Obukhova P.S. ¹ , Knirel Yu.A. ² , Nokel A.Yu. ³ , Bovin N.V. ^4,1

Affiliations

1	Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 ul. Miklukho-Maklaya, Moscow, 117997, Russian Federation
2	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47 Leninsky pr., Moscow, 119991, Russian Federation
3	Semiotik LLC, Moscow, Russian Federation
4	School of Engineering, Computer & Mathematical Sciences, Auckland University of Technology, Auckland 1010, New Zealand

Sepharose matrix without immobilized ligands binds antibodies from human blood serum or immunoglobulin preparations. The eluted antibodies bind bacterial polysaccharides having no structural similarity to agarose (Sepharose is a cross-linked polysaccharide agarose) with a high affinity. It is concluded that the identified antibodies are capable of recognizing spatial rather than linear epitopes of bacterial polysaccharides. This side activity of Sepharose matrix should be taken into account in isolating target antibodies and other proteins from human blood.

Dobrochaeva K.L. , Khasbiullina N.R. , Shilova N.V. , Obukhova P.S. , Knirel Y.A. , Nokel A.Y. , Bovin N.V.
Human antibodies eluted from ligand-free Sepharose capable of binding bacterial polysaccharides and sulfated glycans
Molecular Immunology. 2019. V.106. P.63-68. DOI: 10.1016/j.molimm.2018.12.011 WOS Scopus OpenAlex

Web of science:	WOS:000457507200008
Scopus:	2-s2.0-85058810199
OpenAlex:	W2905869139

DB	Citing
OpenAlex	11
Scopus	11
Web of science	10