Acinetobacter baumannii K20 and K21 capsular polysaccharide structures establish roles for UDP-glucose dehydrogenase Ugd2, pyruvyl transferase Ptr2 and two glycosyltransferases

Acinetobacter baumannii K20 and K21 capsular polysaccharide structures establish roles for UDP-glucose dehydrogenase Ugd2, pyruvyl transferase Ptr2 and two glycosyltransferases Full article

Journal

Glycobiology
ISSN: 1460-2423 , E-ISSN: 0959-6658

Output data

Year: 2018, Volume: 28, Number: 11, Pages: 876-884 Pages count : 9 DOI: 10.1093/glycob/cwy074

Authors

Kasimova Anastasiya A ^1,2 , Kenyon Johanna J ^3,4 , Arbatsky Nikolay P ² , Shashkov Alexander S ² , Popova Anastasiya V ^5,6 , Shneider Mikhail M ⁷ , Knirel Yuriy A ² , Hall Ruth M ⁴

Affiliations

1	Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, 9 Miusskaya pl., Moscow, Russia
2	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47 Leninskii prosp., Moscow, Russia
3	Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, 60 Musk Ave, Kelvin Grove, Brisbane, Australia
4	School of Molecular Bioscience, The University of Sydney, Cnr of Maze Cres and Butlin Ave, Darlington Campus, Sydney, Australia
5	Moscow Institute of Physics and Technology, 9 Institutskii per., Dolgoprudny, Moscow Region, Russia
6	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia
7	M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya ul., Moscow, Russia

Infections caused by Acinetobacter baumannii isolates from the major global clones, GC1 and GC2, are difficult to treat with antibiotics, and phage therapy, which requires extensive knowledge of the variation in the surface polysaccharides, is an option under consideration. The gene clusters directing the synthesis of capsular polysaccharide (CPS) in A. baumannii GC1 isolate A388 and GC2 isolate G21 differ by a single glycosyltransferase (gtr) gene. They include genes encoding a novel UDP-glucose dehydrogenase (Ugd2) and a putative pyruvyl transferase (Ptr2). The composition and structures of the linear K20 and K21 tetrasaccharide repeats (K units) of the CPSs isolated from A338 and G21, respectively, were established by sugar analyses and Smith degradation along with 1D and 2D 1H and 13C NMR spectroscopy. The K20 and K21 CPSs are the first known to include GlcpA produced by Ugd2 and D-galactose with an (R)-configured 4,6-pyruvic acid acetal added by Prt2. The first sugar in the tetrasaccharide K units is 2-acetamido-4-amino-2,4,6-trideoxy-D-glucose (D-QuipNAc4N) that carries a 4-N-[(S)-3-hydroxybutanoyl] group in some K units and a 4-N-acetyl group in the others. Accordingly, K unit polymerases WzyK20 and WzyK21 form a β-D-QuipNAc4NR-(1→2)-D-Galp bond. The K20 and K21 units differ only in the configuration of the glycosidic linkages of D-GlcpNAc allowing the unique inverting glycosyltransferases Gtr43 and the retaining glycosyltransferase Gtr45 to be assigned to the formation of the β-D-GlcpNAc-(1→4)-D-GlcpA and α-D-GlcpNAc-(1→4)-D-GlcpA linkages, respectively.

Kasimova A.A. , Kenyon J.J. , Arbatsky N.P. , Shashkov A.S. , Popova A.V. , Shneider M.M. , Knirel Y.A. , Hall R.M.
Acinetobacter baumannii K20 and K21 capsular polysaccharide structures establish roles for UDP-glucose dehydrogenase Ugd2, pyruvyl transferase Ptr2 and two glycosyltransferases
Glycobiology. 2018. V.28. N11. P.876-884. DOI: 10.1093/glycob/cwy074 WOS Scopus OpenAlex

Web of science:	WOS:000452746400007
Scopus:	2-s2.0-85055074899
OpenAlex:	W2886132070

DB	Citing
OpenAlex	30
Scopus	27