Potential of fluorescence polarization immunoassay for the detection of Aspergillus fumigatus galactomannan

Potential of fluorescence polarization immunoassay for the detection of Aspergillus fumigatus galactomannan Full article

Journal

Russian Chemical Bulletin
ISSN: 1573-9171 , E-ISSN: 1066-5285

Output data

Year: 2019, Volume: 68, Number: 12, Pages: 2365-2369 Pages count : 5 DOI: 10.1007/s11172-019-2713-y

Authors

Krylov V.B. ¹ , Petruk M.I. ^2,1 , Karimova M.P. ² , Mukhametova L.I. ² , Matveev A.L. ³ , Tikunova N.V. ³ , Eremin S.A. ² , Nifantiev N.E. ¹

Affiliations

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47 Leninsky prosp., 119991 Moscow, Russian Federation
2	M. V. Lomonosov Moscow State University, Build 11, 1 Leninskye Gory, 119991 Moscow, Russian Federation
3	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, 8 prosp. Lavrentieva, 630090 Novosibirsk, Russian Federation

Galactomannan (GM) is a specific polysaccharide antigen of opportunistic mold fungi of the genus Aspergillus. The detection of GM in patients’ biological fluids is a reliable criterion for the diagnosis of invasive pulmonary aspergillosis, a severe disease. In this report, we evaluated the analytical capabilities of the fluorescence polarization immunoassay (FPIA) for detection of GM. Using FPIA, the high affinity of the monoclonal antibody 7B8 to the synthetic oligosaccharide β-D-Galf-[(1→5)-β-D-Galf]3-(1→6)-α-D-Manp related to the galactomannan fragment was shown. The binding constant in this interaction was found to be equal to (1.02±0.01) • 108 L mol-1. The detection limit determined for this oligosaccharide with the competitive FPIA method is 3 ng mL-1. The data obtained indicate the possibility of using FPIA for the diagnosis of invasive aspergillosis.

Krylov V.B. , Petruk M.I. , Karimova M.P. , Mukhametova L.I. , Matveev A.L. , Tikunova N.V. , Eremin S.A. , Nifantiev N.E.
Potential of fluorescence polarization immunoassay for the detection of Aspergillus fumigatus galactomannan
Russian Chemical Bulletin. 2019. V.68. N12. P.2365-2369. DOI: 10.1007/s11172-019-2713-y WOS Scopus OpenAlex

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