Structural and serological characterization of the O82 antigen of a Proteus mirabilis strain isolated from a patient in Poland

Structural and serological characterization of the O82 antigen of a Proteus mirabilis strain isolated from a patient in Poland Full article

Journal

Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215

Output data

Year: 2019, Volume: 486, Article number : 107831, Pages count : DOI: 10.1016/j.carres.2019.107831

Authors

Siwińska Małgorzata ¹ , Levina Evgeniya A. ^2,3 , Shashkov Alexander S. ² , Kalinchuk Nadezhda A. ² , Drzewiecka Dominika ¹ , Knirel Yuriy A. ²

Affiliations

1	Laboratory of General Microbiology, Faculty of Biology and Environmental Protection, University of Lodz, 90-237, Lodz, Poland
2	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russia
3	Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, 125047, Moscow, Russia

P. mirabilis strains Kro 45 and Kwy 46 were isolated from the pus and the muscular fluid, respectively, of a hospitalized 61-year-old female in Łódź, Poland. Both strains demonstrated a good swarming ability on a solid medium, and the Dienes test for differentiation of swarming strains indicated their identity. The strains were serologically identical and did not belong to any of the known Proteus O1-O81 serogroups. In this work, we studied the O-specific polysaccharide (O antigen) of P. mirabilis Kwy46, which defines the immunospecificity of the strain. The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide, and the following structure of its oligosaccharide repeat (O-unit) was established by sugar analysis along with 1D and 2D 1H and 13C NMR spectroscopy:

Siwińska M. , Levina E.A. , Shashkov A.S. , Kalinchuk N.A. , Drzewiecka D. , Knirel Y.A.
Structural and serological characterization of the O82 antigen of a Proteus mirabilis strain isolated from a patient in Poland
Carbohydrate Research. 2019. V.486. 107831 . DOI: 10.1016/j.carres.2019.107831 WOS Scopus OpenAlex

Web of science:	WOS:000496174300012
Scopus:	2-s2.0-85073165381
OpenAlex:	W2978825350

DB	Citing
OpenAlex	7
Scopus	6
Web of science	6