Involvement of a multifunctional rhamnosyltransferase in the synthesis of three related Acinetobacter baumannii capsular polysaccharides, K55, K74 and K85

Involvement of a multifunctional rhamnosyltransferase in the synthesis of three related Acinetobacter baumannii capsular polysaccharides, K55, K74 and K85 Full article

Journal

International Journal of Biological Macromolecules
ISSN: 1879-0003 , E-ISSN: 0141-8130

Output data

Year: 2021, Volume: 166, Pages: 1230-1237 Pages count : 8 DOI: 10.1016/j.ijbiomac.2020.11.005

Authors

Kenyon Johanna J. ¹ , Arbatsky Nikolay P. ² , Sweeney Emma L. ¹ , Zhang Yang ³ , Senchenkova Sofya N. ² , Popova Anastasiya V. ^4,5 , Shneider Mikhail M. ⁶ , Shashkov Alexander S. ² , Liu Bin ³ , Hall Ruth M. ⁷ , Knirel Yuriy A. ²

Affiliations

1	Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Queensland University of Technology, Brisbane, Australia
2	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
3	TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, PR China
4	Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia
5	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia
6	M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
7	School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia

KL55, KL74, and KL85 capsular polysaccharide (CPS) biosynthesis loci in Acinetobacter baumannii BAL_204, BAL_309, and LUH5543 genomes, respectively, are related and each contains genes for l-Rhap and d-GlcpA synthesis. The CPSs were isolated and studied by sugar analysis, Smith degradation, and 1H and 13C NMR spectroscopy. The K55 and K74 CPSs are built up of branched octasaccharide repeats (K units) containing one residue each of d-GlcpA and d-GlcpNAc and six residues of l-Rhap. The K55 unit differs from the K74 unit in the linkage between D-GlcpA and an l-Rhap residue in the K unit (1 → 3 versus 1 → 2) and linkage between K units. However, most K units in the isolated K74 CPS were modified by β-elimination of a side-chain α-l-Rhap-(1 → 3)-α-l-Rhap disaccharide from position 4 of GlcA to give 4-deoxy-l-threo-hex-4-enuronic acid (1:~3 ratio of intact and modified units). The K85 CPS has a branched heptasaccharide K unit similar to the K74 unit but with one fewer α-l-Rhap residue in the side chain. In contrast to previous findings on A. baumannii CPSs, each K locus includes fewer glycosyltransferase (Gtr) genes than the number required to form all linkages in the K units. Hence, one Gtr appears to be multifunctional catalysing formation of two 1 → 2 and one 1 → 3 linkages between the l-Rha residues.

Kenyon J.J. , Arbatsky N.P. , Sweeney E.L. , Zhang Y. , Senchenkova S.N. , Popova A.V. , Shneider M.M. , Shashkov A.S. , Liu B. , Hall R.M. , Knirel Y.A.
Involvement of a multifunctional rhamnosyltransferase in the synthesis of three related Acinetobacter baumannii capsular polysaccharides, K55, K74 and K85
International Journal of Biological Macromolecules. 2021. V.166. P.1230-1237. DOI: 10.1016/j.ijbiomac.2020.11.005 WOS Scopus OpenAlex

Web of science:	WOS:000603588400116
Scopus:	2-s2.0-85096131684
OpenAlex:	W3097354319

DB	Citing
OpenAlex	24
Scopus	21
Web of science	21