The K26 capsular polysaccharide from Acinetobacter baumannii KZ-1098: Structure and cleavage by a specific phage depolymerase

The K26 capsular polysaccharide from Acinetobacter baumannii KZ-1098: Structure and cleavage by a specific phage depolymerase Full article

Journal

International Journal of Biological Macromolecules
ISSN: 1879-0003 , E-ISSN: 0141-8130

Output data

Year: 2021, Volume: 191, Pages: 182-191 Pages count : 10 DOI: 10.1016/j.ijbiomac.2021.09.073

Authors

Kasimova Anastasiya A. ¹ , Arbatsky Nikolay P. ¹ , Timoshina Olga Y. ² , Shneider Mikhail M. ² , Shashkov Alexander S. ¹ , Chizhov Alexander O. ¹ , Popova Anastasiya V. ³ , Hall Ruth M. ⁴ , Kenyon Johanna J. ⁵ , Knirel Yuriy A. ²

Affiliations

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia;
2	M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
3	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia
4	School of Life and Environmental Sciences, Faculty of Science, University of Sydney, Sydney, Australia
5	Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology. Brisbane, Australia

The KL26 gene cluster responsible for the synthesis of the K26 capsular polysaccharide (CPS) of Acinetobacter baumannii includes rmlBDAC genes for l-rhamnose (l-Rhap) synthesis, tle to generate 6-deoxy-l-talose (l-6dTalp) from l-Rhap, and a manC gene for D-mannose (D-Manp) that is rare in Acinetobacter CPS. K26 CPS material was isolated from A. baumannii isolate KZ-1098, and studied by sugar analysis, Smith degradation, and one and two-dimensional 1H and 13C NMR spectroscopy before and after O-deacetylation with aqueous ammonia. The following structure of the branched hexasaccharide repeating unit of the CPS was established:

Kasimova A.A. , Arbatsky N.P. , Timoshina O.Y. , Shneider M.M. , Shashkov A.S. , Chizhov A.O. , Popova A.V. , Hall R.M. , Kenyon J.J. , Knirel Y.A.
The K26 capsular polysaccharide from Acinetobacter baumannii KZ-1098: Structure and cleavage by a specific phage depolymerase
International Journal of Biological Macromolecules. 2021. V.191. P.182-191. DOI: 10.1016/j.ijbiomac.2021.09.073 WOS Scopus OpenAlex

Web of science:	WOS:000710169700002
Scopus:	2-s2.0-85115183831
OpenAlex:	W3199409751

DB	Citing
OpenAlex	17
Scopus	16
Web of science	16